Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

Kizee A. Etienne; Chandler C. Roe; Rachel M. Smith; Snigdha Vallabhaneni; Carolina Duarte; Patricia Escandón; Elizabeth Castañeda; Beatriz L. Gómez; Catalina De Bedout; Luisa F. López; Valentina Salas; Luz Maria Hederra; Jorge Fernández; Paola Pidal; Juan Carlos Hormazabel; Fernando Otaíza-O’ryan; Fredrik O. Vannberg; John Gillece; Darrin Lemmer; Elizabeth M. Driebe; David M. Engelthaler; Anastasia P. Litvintseva

doi:10.3201/eid2203.151193

Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

Kizee A. Etienne, Chandler C. Roe, Rachel M. Smith, Snigdha Vallabhaneni, Carolina Duarte, Patricia Escandón, Elizabeth Castañeda, Beatriz L. Gómez, Catalina De Bedout, Luisa F. López, Valentina Salas, Luz Maria Hederra, Jorge Fernández, Paola Pidal, Juan Carlos Hormazabel, Fernando Otaíza-O’ryan, Fredrik O. Vannberg, John Gillece, Darrin Lemmer, Elizabeth M. DriebeDavid M. Engelthaler, Anastasia P. Litvintseva

School of Medicine and Health Sciences

Research output: Contribution to journal › Research Article › peer-review

39 Scopus citations

Abstract

We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.

Original language	English (US)
Pages (from-to)	476-481
Number of pages	6
Journal	Emerging Infectious Diseases
Volume	22
Issue number	3
DOIs	https://doi.org/10.3201/eid2203.151193
State	Published - Mar 2016

All Science Journal Classification (ASJC) codes

Epidemiology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Etienne, K. A., Roe, C. C., Smith, R. M., Vallabhaneni, S., Duarte, C., Escandón, P., Castañeda, E., Gómez, B. L., Bedout, C. D., López, L. F., Salas, V., Hederra, L. M., Fernández, J., Pidal, P., Hormazabel, J. C., Otaíza-O’ryan, F., Vannberg, F. O., Gillece, J., Lemmer, D., ... Litvintseva, A. P. (2016). Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections. Emerging Infectious Diseases, 22(3), 476-481. https://doi.org/10.3201/eid2203.151193

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title = "Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections",

abstract = "We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.",

author = "Etienne, {Kizee A.} and Roe, {Chandler C.} and Smith, {Rachel M.} and Snigdha Vallabhaneni and Carolina Duarte and Patricia Escand{\'o}n and Elizabeth Casta{\~n}eda and G{\'o}mez, {Beatriz L.} and Bedout, {Catalina De} and L{\'o}pez, {Luisa F.} and Valentina Salas and Hederra, {Luz Maria} and Jorge Fern{\'a}ndez and Paola Pidal and Hormazabel, {Juan Carlos} and Fernando Ota{\'i}za-O{\textquoteright}ryan and Vannberg, {Fredrik O.} and John Gillece and Darrin Lemmer and Driebe, {Elizabeth M.} and Engelthaler, {David M.} and Litvintseva, {Anastasia P.}",

year = "2016",

month = mar,

doi = "10.3201/eid2203.151193",

language = "English (US)",

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Etienne, KA, Roe, CC, Smith, RM, Vallabhaneni, S, Duarte, C, Escandón, P, Castañeda, E, Gómez, BL, Bedout, CD, López, LF, Salas, V, Hederra, LM, Fernández, J, Pidal, P, Hormazabel, JC, Otaíza-O’ryan, F, Vannberg, FO, Gillece, J, Lemmer, D, Driebe, EM, Engelthaler, DM & Litvintseva, AP 2016, 'Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections', Emerging Infectious Diseases, vol. 22, no. 3, pp. 476-481. https://doi.org/10.3201/eid2203.151193

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T1 - Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

AU - Etienne, Kizee A.

AU - Roe, Chandler C.

AU - Smith, Rachel M.

AU - Vallabhaneni, Snigdha

AU - Duarte, Carolina

AU - Escandón, Patricia

AU - Castañeda, Elizabeth

AU - Gómez, Beatriz L.

AU - Bedout, Catalina De

AU - López, Luisa F.

AU - Salas, Valentina

AU - Hederra, Luz Maria

AU - Fernández, Jorge

AU - Pidal, Paola

AU - Hormazabel, Juan Carlos

AU - Otaíza-O’ryan, Fernando

AU - Vannberg, Fredrik O.

AU - Gillece, John

AU - Lemmer, Darrin

AU - Driebe, Elizabeth M.

AU - Engelthaler, David M.

AU - Litvintseva, Anastasia P.

PY - 2016/3

Y1 - 2016/3

N2 - We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.

AB - We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.

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M3 - Research Article

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SN - 1080-6040

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JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

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ER -

Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections

Abstract

All Science Journal Classification (ASJC) codes

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