TY - JOUR
T1 - Whole-genome sequencing to determine origin of multinational outbreak of Sarocladium kiliense bloodstream infections
AU - Etienne, Kizee A.
AU - Roe, Chandler C.
AU - Smith, Rachel M.
AU - Vallabhaneni, Snigdha
AU - Duarte, Carolina
AU - Escandón, Patricia
AU - Castañeda, Elizabeth
AU - Gómez, Beatriz L.
AU - Bedout, Catalina De
AU - López, Luisa F.
AU - Salas, Valentina
AU - Hederra, Luz Maria
AU - Fernández, Jorge
AU - Pidal, Paola
AU - Hormazabel, Juan Carlos
AU - Otaíza-O’ryan, Fernando
AU - Vannberg, Fredrik O.
AU - Gillece, John
AU - Lemmer, Darrin
AU - Driebe, Elizabeth M.
AU - Engelthaler, David M.
AU - Litvintseva, Anastasia P.
N1 - Publisher Copyright:
© 2016, Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2016/3
Y1 - 2016/3
N2 - We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.
AB - We used whole-genome sequence typing (WGST) to investigate an outbreak of Sarocladium kiliense bloodstream infections (BSI) associated with receipt of contaminated antinausea medication among oncology patients in Colombia and Chile during 2013-2014. Twenty-five outbreak isolates (18 from patients and 7 from medication vials) and 11 control isolates unrelated to this outbreak were subjected to WGST to elucidate a source of infection. All outbreak isolates were nearly indistinguishable (≤5 single-nucleotide polymorphisms), and >21,000 single-nucleotide polymorphisms were identified from unrelated control isolates, suggesting a point source for this outbreak. S. kiliense has been previously implicated in healthcare-related infections; however, the lack of available typing methods has precluded the ability to substantiate point sources. WGST for outbreak investigation caused by eukaryotic pathogens without reference genomes or existing genotyping methods enables accurate source identification to guide implementation of appropriate control and prevention measures.
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U2 - 10.3201/eid2203.151193
DO - 10.3201/eid2203.151193
M3 - Article
C2 - 26891230
AN - SCOPUS:84958740885
SN - 1080-6040
VL - 22
SP - 476
EP - 481
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 3
ER -
