TY - JOUR
T1 - Peptidorhamnomannan negatively modulates the immune response in a scedosporiosis murine model
AU - Xisto, Mariana I.D.S.
AU - Liporagi-Lopes, Livia Cristina
AU - Muñoz, Julián Esteban
AU - Bittencourt, Vera C.B.
AU - Santos, Giulia M.P.
AU - Dias, Lucas S.
AU - Figueiredo, Rodrigo T.
AU - Pinto, Márcia R.
AU - Taborda, Carlos P.
AU - Barreto-Bergter, Eliana
N1 - Funding Information:
We are grateful to Maria Isabel Doria Rossi, from the Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro at Rio de Janeiro, Brazil, for helpful in the histological analysis on the manuscript. This work was supported by grants from the Conselho Nacional de esenvolvimentoCientífico e Tecnológico (CNPq), Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - In this study, we analyzed the impact of immunization with the peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium (Lomentospora) prolificans in a murine model of invasive scedosporiosis. Immunization with PRM decreased the survival of mice infected with S. prolificans. Immunization of mice with PRM led to decreased secretion of pro-inflammatory cytokines and chemokines but did not affect the secretion of IL-10. Mice immunized with PRM showed an increase in IgG1 secretion, which is an immunoglobulin linked to a nonprotective response. Splenocytes isolated from mice infected with S. prolificans and immunized with PRM showed no differences in the percentages of Th17 cells and no increase in the frequency of the CD4+CD62LLow T cell population. PRM-immunized mice showed a significant increase in the percentage of Treg cells. In summary, our results indicated that immunization with PRM did not assist or improve the immunological response against S. prolificans infection. PRM exacerbated the infection process by reducing the inflammatory response, thereby facilitating colonization, virulence and dissemination by the fungus.
AB - In this study, we analyzed the impact of immunization with the peptidorhamnomannan (PRM) from the cell wall of the fungus Scedosporium (Lomentospora) prolificans in a murine model of invasive scedosporiosis. Immunization with PRM decreased the survival of mice infected with S. prolificans. Immunization of mice with PRM led to decreased secretion of pro-inflammatory cytokines and chemokines but did not affect the secretion of IL-10. Mice immunized with PRM showed an increase in IgG1 secretion, which is an immunoglobulin linked to a nonprotective response. Splenocytes isolated from mice infected with S. prolificans and immunized with PRM showed no differences in the percentages of Th17 cells and no increase in the frequency of the CD4+CD62LLow T cell population. PRM-immunized mice showed a significant increase in the percentage of Treg cells. In summary, our results indicated that immunization with PRM did not assist or improve the immunological response against S. prolificans infection. PRM exacerbated the infection process by reducing the inflammatory response, thereby facilitating colonization, virulence and dissemination by the fungus.
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U2 - 10.1093/mmy/myw039
DO - 10.1093/mmy/myw039
M3 - Article
C2 - 27343286
AN - SCOPUS:84994559192
SN - 1369-3786
VL - 54
SP - 846
EP - 855
JO - Medical Mycology
JF - Medical Mycology
IS - 8
ER -
