TY - JOUR
T1 - Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions
AU - Pinzon-Daza, Martha L.
AU - Cuellar-Saenz, Yenith
AU - Nualart, Francisco
AU - Ondo-Mendez, Alejandro
AU - Del Riesgo, Lilia
AU - Castillo-Rivera, Fabio
AU - Garzon, Ruth
PY - 2017/7
Y1 - 2017/7
N2 - P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors
AB - P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors
U2 - 10.1002/jcb.25890
DO - 10.1002/jcb.25890
M3 - Artículo
C2 - 28106284
SN - 0730-2312
VL - 118
SP - 1868
EP - 1878
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
IS - 7
M1 - 7
ER -
