Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions

Martha L. Pinzon-Daza; Yenith Cuellar-Saenz; Francisco Nualart; Alejandro Ondo-Mendez; Lilia Del Riesgo; Fabio Castillo-Rivera; Ruth Garzon

doi:10.1002/jcb.25890

Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions

Martha L. Pinzon-Daza, Yenith Cuellar-Saenz, Francisco Nualart, Alejandro Ondo-Mendez, Lilia Del Riesgo, Fabio Castillo-Rivera, Ruth Garzon

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

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Resumen

P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors

Idioma original	Inglés
Número de artículo	7
Páginas (desde-hasta)	1868-1878
Número de páginas	10
Publicación	Journal of Cellular Biochemistry
Volumen	118
N.º	7
DOI	https://doi.org/10.1002/jcb.25890
Estado	Publicada - jul. 2017

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title = "Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions",

abstract = "P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors",

author = "Pinzon-Daza, {Martha L.} and Yenith Cuellar-Saenz and Francisco Nualart and Alejandro Ondo-Mendez and {Del Riesgo}, Lilia and Fabio Castillo-Rivera and Ruth Garzon",

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Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions. / Pinzon-Daza, Martha L.; Cuellar-Saenz, Yenith; Nualart, Francisco et al.
En: Journal of Cellular Biochemistry, Vol. 118, N.º 7, 7, 07.2017, p. 1868-1878.

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - Oxidative Stress Promotes Doxorubicin-Induced Pgp and BCRP Expression in Colon Cancer Cells Under Hypoxic Conditions

AU - Pinzon-Daza, Martha L.

AU - Cuellar-Saenz, Yenith

AU - Nualart, Francisco

AU - Ondo-Mendez, Alejandro

AU - Del Riesgo, Lilia

AU - Castillo-Rivera, Fabio

AU - Garzon, Ruth

PY - 2017/7

Y1 - 2017/7

N2 - P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors

AB - P‐glycoprotein (Pgp) and breast cancer resistance protein (BCRP) are ATP binding cassette (ABC) transporters that are overexpressed in different drug‐resistant cancer cell lines. In this study, we investigated whether doxorubicin promotes Pgp and/or BCRP expression to induce drug resistance in colon cancer cells under hypoxic conditions. We analyzed HIF‐1α activity via ELISA, Pgp, and BCRP expression by qRT‐PCR and the relationship between doxorubicin uptake and ABC transporter expression via confocal microscopy in HT‐29WT and HT‐29 doxorubicin‐resistant colon cancer cells (HT‐29DxR). These cells were treated with doxorubicin and/or CoCl2 (chemical hypoxia), and reactive oxygen species inductors

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DO - 10.1002/jcb.25890

M3 - Artículo de Investigación

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SN - 0730-2312

VL - 118

SP - 1868

EP - 1878

JO - Journal of Cellular Biochemistry

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