TY - JOUR
T1 - CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis
AU - García-López, Laura Lorena
AU - Vargas-Montes, Mónica
AU - Osorio-Méndez, Juan Felipe
AU - Cardona, Néstor
AU - Hernández De Los Ríos, Alejandro
AU - Toro-Acevedo, Carlos Andrés
AU - Arenas-García, Juan Camilo
AU - Mantilla-Muriel, Luz Eliana
AU - Torres, Elizabeth
AU - Valencia-Hernández, Juan D.
AU - Acosta-Dávila, Alejandro
AU - de-la-Torre, Alejandra
AU - Celis-Giraldo, Daniel
AU - Mejía Oquendo, Manuela
AU - Sepúlveda-Arias, Juan Carlos
AU - Gómez-Marín, Jorge Enrique
N1 - Publisher Copyright:
© 2023 Taylor & Francis Group, LLC.
PY - 2023/6/14
Y1 - 2023/6/14
N2 - This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.
AB - This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.
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U2 - 10.1080/09273948.2023.2217906
DO - 10.1080/09273948.2023.2217906
M3 - Research Article
C2 - 37315178
AN - SCOPUS:85161923043
SN - 0927-3948
JO - Ocular Immunology and Inflammation
JF - Ocular Immunology and Inflammation
ER -
