CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis

Laura Lorena García-López; Mónica Vargas-Montes; Juan Felipe Osorio-Méndez; Néstor Cardona; Alejandro Hernández De Los Ríos; Carlos Andrés Toro-Acevedo; Juan Camilo Arenas-García; Luz Eliana Mantilla-Muriel; Elizabeth Torres; Juan D. Valencia-Hernández; Alejandro Acosta-Dávila; Alejandra de-la-Torre; Daniel Celis-Giraldo; Manuela Mejía Oquendo; Juan Carlos Sepúlveda-Arias; Jorge Enrique Gómez-Marín

doi:10.1080/09273948.2023.2217906

CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis

Laura Lorena García-López, Mónica Vargas-Montes, Juan Felipe Osorio-Méndez, Néstor Cardona, Alejandro Hernández De Los Ríos, Carlos Andrés Toro-Acevedo, Juan Camilo Arenas-García, Luz Eliana Mantilla-Muriel, Elizabeth Torres, Juan D. Valencia-Hernández, Alejandro Acosta-Dávila, Alejandra de-la-Torre, Daniel Celis-Giraldo, Manuela Mejía Oquendo, Juan Carlos Sepúlveda-Arias, Jorge Enrique Gómez-Marín

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

2 Citas (Scopus)

Resumen

En este trabajo se analizaron marcadores de agotamiento en subpoblaciones de células T CD8+ en 21 muestras de células mononucleares de sangre periférica (PBMC) de individuos con toxoplasmosis ocular (n = 9), toxoplasmosis crónica asintomática (n = 7) y no infectados (n = 5) mediante técnicas de RT-qPCR y citometría de flujo. El estudio halló que la expresión génica de PD-1 y CD244, pero no de LAG-3, era mayor en individuos con toxoplasmosis ocular frente a individuos con infección asintomática o no infectados. La expresión de PD1 en células CD8+ de memoria central (CM) fue mayor en nueve individuos con toxoplasmosis frente a cinco individuos no infectados (p = 0,003). Tras la estimulación ex vivo, se encontró una correlación inversa entre los marcadores de agotamiento y las características clínicas cuantitativas (tamaño de la lesión, índice de recurrencia y número de lesiones). Se encontró un fenotipo de agotamiento total en el 55,5% (5/9) de los individuos con toxoplasmosis ocular. Nuestros resultados sugieren que el fenotipo de agotamiento CD8+ está implicado en la patogénesis de la toxoplasmosis ocular.

Idioma original	Inglés estadounidense
Publicación	Ocular Immunology and Inflammation
DOI	https://doi.org/10.1080/09273948.2023.2217906
Estado	Publicada - jun. 14 2023

Áreas temáticas de ASJC Scopus

Inmulogía y alergología
Oftalmología

ODS de las Naciones Unidas

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García-López, L. L., Vargas-Montes, M., Osorio-Méndez, J. F., Cardona, N., Hernández De Los Ríos, A., Toro-Acevedo, C. A., Arenas-García, J. C., Mantilla-Muriel, L. E., Torres, E., Valencia-Hernández, J. D., Acosta-Dávila, A., de-la-Torre, A., Celis-Giraldo, D., Mejía Oquendo, M., Sepúlveda-Arias, J. C., & Gómez-Marín, J. E. (2023). CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis. Ocular Immunology and Inflammation. https://doi.org/10.1080/09273948.2023.2217906

@article{0e63fd9239cc49ce878e4271d7be4bdd,

title = "CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis",

abstract = "This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.",

author = "Garc{\'i}a-L{\'o}pez, {Laura Lorena} and M{\'o}nica Vargas-Montes and Osorio-M{\'e}ndez, {Juan Felipe} and N{\'e}stor Cardona and {Hern{\'a}ndez De Los R{\'i}os}, Alejandro and Toro-Acevedo, {Carlos Andr{\'e}s} and Arenas-Garc{\'i}a, {Juan Camilo} and Mantilla-Muriel, {Luz Eliana} and Elizabeth Torres and Valencia-Hern{\'a}ndez, {Juan D.} and Alejandro Acosta-D{\'a}vila and Alejandra de-la-Torre and Daniel Celis-Giraldo and {Mej{\'i}a Oquendo}, Manuela and Sep{\'u}lveda-Arias, {Juan Carlos} and G{\'o}mez-Mar{\'i}n, {Jorge Enrique}",

note = "Publisher Copyright: {\textcopyright} 2023 Taylor & Francis Group, LLC.",

year = "2023",

month = jun,

day = "14",

doi = "10.1080/09273948.2023.2217906",

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García-López, LL, Vargas-Montes, M, Osorio-Méndez, JF, Cardona, N, Hernández De Los Ríos, A, Toro-Acevedo, CA, Arenas-García, JC, Mantilla-Muriel, LE, Torres, E, Valencia-Hernández, JD, Acosta-Dávila, A, de-la-Torre, A, Celis-Giraldo, D, Mejía Oquendo, M, Sepúlveda-Arias, JC & Gómez-Marín, JE 2023, 'CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis', Ocular Immunology and Inflammation. https://doi.org/10.1080/09273948.2023.2217906

TY - JOUR

T1 - CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis

AU - García-López, Laura Lorena

AU - Vargas-Montes, Mónica

AU - Osorio-Méndez, Juan Felipe

AU - Cardona, Néstor

AU - Hernández De Los Ríos, Alejandro

AU - Toro-Acevedo, Carlos Andrés

AU - Arenas-García, Juan Camilo

AU - Mantilla-Muriel, Luz Eliana

AU - Torres, Elizabeth

AU - Valencia-Hernández, Juan D.

AU - Acosta-Dávila, Alejandro

AU - de-la-Torre, Alejandra

AU - Celis-Giraldo, Daniel

AU - Mejía Oquendo, Manuela

AU - Sepúlveda-Arias, Juan Carlos

AU - Gómez-Marín, Jorge Enrique

PY - 2023/6/14

Y1 - 2023/6/14

N2 - This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.

AB - This work analyzed exhaustion markers in CD8+ T-cell subpopulations in 21 samples of peripheral blood mononuclear cells (PBMCs) from individuals with ocular toxoplasmosis (n = 9), chronic asymptomatic toxoplasmosis (n = 7), and non-infected people (n = 5) by using RT-qPCR and flow cytometry techniques. The study found that gene expression of PD-1 and CD244, but not LAG-3, was higher in individuals with ocular toxoplasmosis versus individuals with asymptomatic infection or uninfected. Expression of PD1 in CD8+ central memory (CM) cells was higher in nine individuals with toxoplasmosis versus five uninfected individuals (p =.003). After ex vivo stimulation, an inverse correlation was found between the exhaustion markers and quantitative clinical characteristics (lesion size, recurrence index, and number of lesions). A total exhaustion phenotype was found in 55.5% (5/9) of individuals with ocular toxoplasmosis. Our results suggest that the CD8+ exhaustion phenotype is involved in the pathogenesis of ocular toxoplasmosis.

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U2 - 10.1080/09273948.2023.2217906

DO - 10.1080/09273948.2023.2217906

M3 - Research Article

C2 - 37315178

AN - SCOPUS:85161923043

SN - 0927-3948

JO - Ocular Immunology and Inflammation

JF - Ocular Immunology and Inflammation

ER -

CD8+ T–cell Exhaustion Phenotype in Human Asymptomatic and Ocular Toxoplasmosis

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