Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC

Adriana Janneth Bermudez Quintero, Carlos Giovanni Pinzón, Hernando Curtidor, Martha Forero, Magnolia Vanegas Murcia, Jorge Rodríguez, Manuel Elkin Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

17 Citas (Scopus)

Resumen

Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparumin vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis
Idioma originalEnglish (US)
Páginas (desde-hasta)853-862
Número de páginas10
PublicaciónVaccine
Volumen26
N.º6
DOI
EstadoPublished - feb 6 2008

Huella dactilar

Plasmodium falciparum
Membrane Proteins
erythrocytes
Erythrocytes
peptides
antigens
Antigens
Peptides
proteins
circular dichroism spectroscopy
Circular Dichroism
rhoptry
receptors
Membranes
assays
Enzymes
enzymes

Citar esto

Bermudez Quintero, A. J., Pinzón, C. G., Curtidor, H., Forero, M., Vanegas Murcia, M., Rodríguez, J., & Patarroyo, M. E. (2008). Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC. Vaccine, 26(6), 853-862. https://doi.org/10.1016/j.vaccine.2007.11.086
Bermudez Quintero, Adriana Janneth ; Pinzón, Carlos Giovanni ; Curtidor, Hernando ; Forero, Martha ; Vanegas Murcia, Magnolia ; Rodríguez, Jorge ; Patarroyo, Manuel Elkin. / Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC. En: Vaccine. 2008 ; Vol. 26, N.º 6. pp. 853-862.
@article{849841a5d0204c0eb5c206870f0eb245,
title = "Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC",
abstract = "Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparumin vitro invasion of normal RBC by up to 61{\%} (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis",
author = "{Bermudez Quintero}, {Adriana Janneth} and Pinz{\'o}n, {Carlos Giovanni} and Hernando Curtidor and Martha Forero and {Vanegas Murcia}, Magnolia and Jorge Rodr{\'i}guez and Patarroyo, {Manuel Elkin}",
year = "2008",
month = "2",
day = "6",
doi = "10.1016/j.vaccine.2007.11.086",
language = "English (US)",
volume = "26",
pages = "853--862",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "6",

}

Bermudez Quintero, AJ, Pinzón, CG, Curtidor, H, Forero, M, Vanegas Murcia, M, Rodríguez, J & Patarroyo, ME 2008, 'Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC', Vaccine, vol. 26, n.º 6, pp. 853-862. https://doi.org/10.1016/j.vaccine.2007.11.086

Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC. / Bermudez Quintero, Adriana Janneth; Pinzón, Carlos Giovanni; Curtidor, Hernando; Forero, Martha; Vanegas Murcia, Magnolia; Rodríguez, Jorge; Patarroyo, Manuel Elkin.

En: Vaccine, Vol. 26, N.º 6, 06.02.2008, p. 853-862.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC

AU - Bermudez Quintero, Adriana Janneth

AU - Pinzón, Carlos Giovanni

AU - Curtidor, Hernando

AU - Forero, Martha

AU - Vanegas Murcia, Magnolia

AU - Rodríguez, Jorge

AU - Patarroyo, Manuel Elkin

PY - 2008/2/6

Y1 - 2008/2/6

N2 - Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparumin vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis

AB - Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparumin vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis

UR - http://www.sciencedirect.com/science/article/pii/S0264410X07013072?via%3Dihub

U2 - 10.1016/j.vaccine.2007.11.086

DO - 10.1016/j.vaccine.2007.11.086

M3 - Article

VL - 26

SP - 853

EP - 862

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 6

ER -

Bermudez Quintero AJ, Pinzón CG, Curtidor H, Forero M, Vanegas Murcia M, Rodríguez J y otros. Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC. Vaccine. 2008 feb 6;26(6):853-862. https://doi.org/10.1016/j.vaccine.2007.11.086