Studies of Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) protein peptides specifically binding to human RBC

Adriana Janneth Bermudez Quintero, Carlos Giovanni Pinzón, Hernando Curtidor Castellanos, Martha Forero, Magnolia Vanegas Murcia, Jorge Rodríguez, Manuel Elkin Patarroyo

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Plasmodium falciparum rhoptry-associated membrane antigen (RAMA) peptides used in normal red blood cell (RBC) binding assays revealed that peptides 33426 (79NINILSSVHRKGRILYDSF97) and 33460 (777HKKREKSISPHSYQKVSTKVQ797) bound with high activity, presenting nanomolar affinity constants. Such high binding activity peptides (HABPs) displayed helicoid and random coil structures as determined by circular dichroism. HABPs inhibited P. falciparumin vitro invasion of normal RBC by up to 61% (depending on concentration), suggesting that some RAMA protein regions could be involved in P. falciparum invasion of RBC. The nature and localisation of receptors on RBC surface responsible for HABP binding were studied using enzyme-treated erythrocytes and structural analysis
Original languageEnglish (US)
Pages (from-to)853-862
Number of pages10
JournalVaccine
Volume26
Issue number6
DOIs
StatePublished - Feb 6 2008

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