Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Adriana Janneth Bermudez Quintero; Javier E García; Alvaro Puentes; Ramsés López; Ricardo Vera; Jorge Suárez; Hernando Curtidor; Luis Eduardo Rodriguez; Marisol Ocampo Cifuentes; Diana R. Tovar; Martha Forero; Jimena Cortés; Mauricio Urquiza; Manuel Elkin Patarroyo

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Adriana Janneth Bermudez Quintero, Javier E García, Alvaro Puentes, Ramsés López, Ricardo Vera, Jorge Suárez, Hernando Curtidor, Luis Eduardo Rodriguez, Marisol Ocampo Cifuentes, Diana R. Tovar, Martha Forero, Jimena Cortés, Mauricio Urquiza, Manuel Elkin Patarroyo

Escuela de Medicina y Ciencias de la Salud

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

17 Citas (Scopus)

Resumen

Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)), 20637 ((157)KEKLQGQQSDSEQERRAY(173)), 20638 ((174)KEKLQEQQSDLEQERLAY(190)) and 20639 (191KEKLQEQQSDLEQERRAY(207)) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)) and 20633 ((81)DKELTMSNVKNVSQTNFKSLY(100)) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

Idioma original	Inglés estadounidense
Páginas (desde-hasta)	647-657
Número de páginas	11
Publicación	Peptides
Volumen	24
N.º	5
Estado	Publicada - sep. 2003

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Bermudez Quintero, A. J., García, J. E., Puentes, A., López, R., Vera, R., Suárez, J., Curtidor, H., Rodriguez, L. E., Ocampo Cifuentes, M., Tovar, D. R., Forero, M., Cortés, J., Urquiza, M., & Patarroyo, M. E. (2003). Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes. Peptides, 24(5), 647-657. https://www.sciencedirect.com/science/article/abs/pii/S0196978103001359

@article{3e3709d86ab84ad1b985a706be6eefb6,

title = "Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes",

abstract = "Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)), 20637 ((157)KEKLQGQQSDSEQERRAY(173)), 20638 ((174)KEKLQEQQSDLEQERLAY(190)) and 20639 (191KEKLQEQQSDLEQERRAY(207)) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)) and 20633 ((81)DKELTMSNVKNVSQTNFKSLY(100)) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 {"}High Activity Binding Peptides{"} could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.",

author = "{Bermudez Quintero}, {Adriana Janneth} and Garc{\'i}a, {Javier E} and Alvaro Puentes and Rams{\'e}s L{\'o}pez and Ricardo Vera and Jorge Su{\'a}rez and Hernando Curtidor and Rodriguez, {Luis Eduardo} and {Ocampo Cifuentes}, Marisol and Tovar, {Diana R.} and Martha Forero and Jimena Cort{\'e}s and Mauricio Urquiza and Patarroyo, {Manuel Elkin}",

year = "2003",

month = sep,

language = "English (US)",

volume = "24",

pages = "647--657",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "5",

}

Bermudez Quintero, AJ, García, JE, Puentes, A, López, R, Vera, R, Suárez, J, Curtidor, H, Rodriguez, LE, Ocampo Cifuentes, M, Tovar, DR, Forero, M, Cortés, J, Urquiza, M & Patarroyo, ME 2003, 'Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes', Peptides, vol. 24, n.º 5, pp. 647-657. <https://www.sciencedirect.com/science/article/abs/pii/S0196978103001359>

TY - JOUR

T1 - Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

AU - Bermudez Quintero, Adriana Janneth

AU - García, Javier E

AU - Puentes, Alvaro

AU - López, Ramsés

AU - Vera, Ricardo

AU - Suárez, Jorge

AU - Curtidor, Hernando

AU - Rodriguez, Luis Eduardo

AU - Ocampo Cifuentes, Marisol

AU - Tovar, Diana R.

AU - Forero, Martha

AU - Cortés, Jimena

AU - Urquiza, Mauricio

AU - Patarroyo, Manuel Elkin

PY - 2003/9

Y1 - 2003/9

N2 - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)), 20637 ((157)KEKLQGQQSDSEQERRAY(173)), 20638 ((174)KEKLQEQQSDLEQERLAY(190)) and 20639 (191KEKLQEQQSDLEQERRAY(207)) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)) and 20633 ((81)DKELTMSNVKNVSQTNFKSLY(100)) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

AB - Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)), 20637 ((157)KEKLQGQQSDSEQERRAY(173)), 20638 ((174)KEKLQEQQSDLEQERLAY(190)) and 20639 (191KEKLQEQQSDLEQERRAY(207)) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)) and 20633 ((81)DKELTMSNVKNVSQTNFKSLY(100)) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

M3 - Article

C2 - 12895649

SN - 0196-9781

VL - 24

SP - 647

EP - 657

JO - Peptides

JF - Peptides

IS - 5

ER -

Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

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