Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes

Adriana Janneth Bermudez Quintero, Javier E García, Alvaro Puentes, Ramsés López, Ricardo Vera, Jorge Suárez, Hernando Curtidor, Luis Eduardo Rodriguez, Marisol Ocampo Cifuentes, Diana R. Tovar, Martha Forero, Jimena Cortés, Mauricio Urquiza, Manuel Elkin Patarroyo

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Synthetic peptides from the liver stage antigen-1 (LSA-1) antigen sequence were used in HepG2 cell and erythrocyte binding assays to identify regions that could be involved in parasite invasion. LSA-1 protein peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)), 20637 ((157)KEKLQGQQSDSEQERRAY(173)), 20638 ((174)KEKLQEQQSDLEQERLAY(190)) and 20639 (191KEKLQEQQSDLEQERRAY(207)) had high binding activity in HepG2 assays. Were located in immunogenic regions; peptide cell binding was saturable. Peptide 20630 bound specifically to 48kDa HepG2 membrane surface protein. LSA-1 peptides 20630 ((21)INGKIIKNSEKDEIIKSNLRY(40)) and 20633 ((81)DKELTMSNVKNVSQTNFKSLY(100)) showed specific erythrocyte binding activity and inhibited merozoite invasion of erythrocytes in vitro. A monkey serum prepared against LSA-1 20630 peptide analog (CGINGKNIKNAEKPMIIKSNLRGC) inhibited merozoite invasion in vitro. The data suggest LSA-1 "High Activity Binding Peptides" could play a possible role in hepatic cell invasion as well as merozoite invasion of erythrocytes.

Original languageEnglish (US)
Pages (from-to)647-657
Number of pages11
JournalPeptides
Volume24
Issue number5
StatePublished - Sep 2003

Fingerprint Dive into the research topics of 'Peptides of the liver stage antigen-1 (LSA-1) of Plasmodium falciparum bind to human hepatocytes'. Together they form a unique fingerprint.

Cite this