Abstract
Invasive fungal infections (IFIs) caused by Candida species are an emerging threat globally, given that patients at-risk and antifungal resistance are increasing. Antimicrobial peptides (AMPs) have shown good therapeutic capacity against different multidrug-resistant (MDR) microorganisms. This study evaluated the activity of the synthetic peptide, PNR20, against Candida albicans ATCC 10231 and a MDR Colombian clinical isolate of Candida auris. Perturbation of yeast cell surface was evaluated using scanning electron microscopy. Cell viability of Vero cells was determined to assess peptide toxicity. Additionally, survival, fungal burden, and histopathology of BALB/c mice infected intravenously with each Candida species and treated with PNR20 were analyzed. Morphological alterations were identified in both species, demonstrating the antifungal effect of PNR20. In vitro, Vero cells’ viability was not affected by PNR20. All mice infected with either C. albicans or C. auris and treated with PNR20 survived and had a significant reduction in the fungal burden in the kidney compared to the control group. The histopathological analysis in mice infected and treated with PNR20 showed more preserved tissues, without the presence of yeast, compared to the control groups. This work shows that the utilization of PNR20 is a promising therapeutic alternative against disseminated candidiasis.
Translated title of the contribution | Uso terapéutico del péptido antimicrobiano PNR20 para resolver la candidiasis diseminada en un modelo murino |
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Original language | English (US) |
Article number | 1149 |
Pages (from-to) | 1-14 |
Number of pages | 14 |
Journal | Journal of Fungi |
Volume | 9 |
Issue number | 12 |
DOIs | |
State | Published - Nov 28 2023 |
All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Microbiology (medical)
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Mejor Poster Ciencias Básicas - 21 Día de la Investigación
Munoz Henao, Julian Esteban (Recipient) & Micelly, Jeisson Estiven (Recipient), Nov 17 2023
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