TY - JOUR
T1 - The Plasmodium vivax Pv41 surface protein
T2 - Identification and characterization
AU - Angel, Diana I.
AU - Mongui, Alvaro
AU - Ardila, Javier
AU - Vanegas, Magnolia
AU - Patarroyo, Manuel A.
N1 - Funding Information:
We thank Gina Gallego and Diego Garzón for their technical support. We would also like to thank Nora Martinez for reviewing this manuscript. Special gratitude goes to Professor Manuel Elkin Patarroyo for his invaluable comments and suggestions. This research was supported by President of Colombia’s office and the “Instituto Colombiano para el Desarrollo de la Ciencia ‘Francisco Jose de Caldas’ (COLCIENCIAS)” Contract RC-2008.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/12/26
Y1 - 2008/12/26
N2 - Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface.
AB - Recently, Plasmodium vivax has been related to nearly 81% of malaria cases reported in Central America and the Mediterranean. Due to the difficulty of culturing this parasite species in vitro, most studies on P. vivax have focused on the identification of new antigens by homology comparison with P. falciparum vaccine candidate proteins. In this study, we have identified and characterized a Pf41 homologue in P. vivax, hence named Pv41, by following such approach and using web-available bioinformatics databases, molecular techniques and immunochemistry assays. Pv41 protein is a 384-amino-acid-long antigen encoded by a single exon that exhibits two s48/45 domains characteristic of gametocyte surface proteins. We have also demonstrated Pv41 transcription and expression during late intra-erythrocytic parasite stages and defined its subcellular localization on the parasite surface.
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U2 - 10.1016/j.bbrc.2008.10.129
DO - 10.1016/j.bbrc.2008.10.129
M3 - Article
C2 - 18983983
AN - SCOPUS:56349090489
SN - 0006-291X
VL - 377
SP - 1113
EP - 1117
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
