Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose

J. Bustos; P. Zamora; E. Mejía; Y. Varela; B. Gómez

doi:10.1007/BF01314039

Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose

J. Bustos, P. Zamora, E. Mejía, Y. Varela, B. Gómez

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

Original language	English (US)
Pages (from-to)	285-288
Number of pages	4
Journal	Archives of Virology
Volume	118
Issue number	3-4
DOIs	https://doi.org/10.1007/BF01314039
State	Published - Sep 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

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10.1007/BF01314039

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@article{ab90c3e0626a4f1589d04356ab3780e5,

title = "Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose",

abstract = "Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.",

author = "J. Bustos and P. Zamora and E. Mej{\'i}a and Y. Varela and B. G{\'o}mez",

year = "1991",

month = sep,

doi = "10.1007/BF01314039",

language = "English (US)",

volume = "118",

pages = "285--288",

journal = "Archives of Virology",

issn = "0304-8608",

publisher = "Springer-Verlag Wien",

number = "3-4",

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T1 - Purification of rubella virus by isopycnic gradients

T2 - continuous Percoll versus discontinuous sucrose

AU - Bustos, J.

AU - Zamora, P.

AU - Mejía, E.

AU - Varela, Y.

AU - Gómez, B.

PY - 1991/9

Y1 - 1991/9

N2 - Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

AB - Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

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JO - Archives of Virology

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Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose

Abstract

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