Abstract
The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.
Translated title of the contribution | Prueba molecular Genotype®MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente |
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Original language | English (US) |
Pages (from-to) | 92-98 |
Number of pages | 7 |
Journal | Revista Peruana de Medicina Experimental y Salud Publica |
Volume | 29 |
Issue number | 1 |
DOIs | |
State | Published - Mar 2012 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Public Health, Environmental and Occupational Health