Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis

Luis Asencios, Marco Galarza, Neyda Quispe, Lucy Vásquez, Elena Leo, Eddy Valencia, Juan Ramírez, Margoth Acurio, Rosario Salazar, Alberto Mendoza-Ticona, Omar Cáceres

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.

Translated title of the contributionPrueba molecular Genotype®MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente
Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
JournalRevista Peruana de Medicina Experimental y Salud Publica
Issue number1
StatePublished - Mar 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health


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