Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022

Luz H. Patiño, Susana Guerra, Marina Muñoz, Nicolas Luna, Keith Farrugia, Adriana van de Guchte, Zain Khalil, Ana Silvia Gonzalez-Reiche, Matthew M. Hernandez, Radhika Banu, Paras Shrestha, Bernadette Liggayu, Adolfo Firpo Betancourt, David Reich, Carlos Cordon-Cardo, Randy Albrecht, Rebecca Pearl, Viviana Simon, Aria Rooker, Emilia Mia SordilloHarm van Bakel, Adolfo García-Sastre, Dusan Bogunovic, Gustavo Palacios, Alberto Paniz Mondolfi, Juan David Ramírez

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Monkeypox (MPOX) is a zoonotic disease endemic to regions of Central/Western Africa. The geographic endemicity of MPV has expanded, broadening the human-monkeypox virus interface and its potential for spillover. Since May 2022, a large multi-country MPV outbreak with no proven links to endemic countries has originated in Europe and has rapidly expanded around the globe, setting off genomic surveillance efforts. Here, we conducted a genomic analysis of 23 MPV-infected patients from New York City during the early outbreak, assessing the phylogenetic relationship of these strains against publicly available MPV genomes. Additionally, we compared the genomic sequences of clinical isolates versus culture-passaged samples from a subset of samples. Phylogenetic analysis revealed that MPV genomes included in this study cluster within the B.1 lineage (Clade IIb), with some of the samples displaying further differentiation into five different sub-lineages of B.1. Mutational analysis revealed 55 non-synonymous polymorphisms throughout the genome, with some of these mutations located in critical regions required for viral multiplication, structural and assembly functions, as well as the target region for antiviral treatment. In addition, we identified a large majority of polymorphisms associated with GA > AA and TC > TT nucleotide replacements, suggesting the action of human APOBEC3 enzyme. A comparison between clinical isolates and cell culture-passaged samples failed to reveal any difference. Our results provide a first glance at the mutational landscape of early MPV-2022 (B.1) circulating strains in NYC.

Original languageEnglish (US)
Article numbere2192830
JournalEmerging Microbes and Infections
Volume12
Issue number1
DOIs
StatePublished - 2023

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Parasitology
  • Microbiology
  • Immunology
  • Drug Discovery
  • Infectious Diseases
  • Virology

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