TY - JOUR
T1 - Phylogenetic landscape of Monkeypox Virus (MPV) during the early outbreak in New York City, 2022
AU - Patiño, Luz H.
AU - Guerra, Susana
AU - Muñoz, Marina
AU - Luna, Nicolas
AU - Farrugia, Keith
AU - van de Guchte, Adriana
AU - Khalil, Zain
AU - Gonzalez-Reiche, Ana Silvia
AU - Hernandez, Matthew M.
AU - Banu, Radhika
AU - Shrestha, Paras
AU - Liggayu, Bernadette
AU - Firpo Betancourt, Adolfo
AU - Reich, David
AU - Cordon-Cardo, Carlos
AU - Albrecht, Randy
AU - Pearl, Rebecca
AU - Simon, Viviana
AU - Rooker, Aria
AU - Sordillo, Emilia Mia
AU - van Bakel, Harm
AU - García-Sastre, Adolfo
AU - Bogunovic, Dusan
AU - Palacios, Gustavo
AU - Paniz Mondolfi, Alberto
AU - Ramírez, Juan David
N1 - Publisher Copyright:
© 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.
PY - 2023
Y1 - 2023
N2 - Monkeypox (MPOX) is a zoonotic disease endemic to regions of Central/Western Africa. The geographic endemicity of MPV has expanded, broadening the human-monkeypox virus interface and its potential for spillover. Since May 2022, a large multi-country MPV outbreak with no proven links to endemic countries has originated in Europe and has rapidly expanded around the globe, setting off genomic surveillance efforts. Here, we conducted a genomic analysis of 23 MPV-infected patients from New York City during the early outbreak, assessing the phylogenetic relationship of these strains against publicly available MPV genomes. Additionally, we compared the genomic sequences of clinical isolates versus culture-passaged samples from a subset of samples. Phylogenetic analysis revealed that MPV genomes included in this study cluster within the B.1 lineage (Clade IIb), with some of the samples displaying further differentiation into five different sub-lineages of B.1. Mutational analysis revealed 55 non-synonymous polymorphisms throughout the genome, with some of these mutations located in critical regions required for viral multiplication, structural and assembly functions, as well as the target region for antiviral treatment. In addition, we identified a large majority of polymorphisms associated with GA > AA and TC > TT nucleotide replacements, suggesting the action of human APOBEC3 enzyme. A comparison between clinical isolates and cell culture-passaged samples failed to reveal any difference. Our results provide a first glance at the mutational landscape of early MPV-2022 (B.1) circulating strains in NYC.
AB - Monkeypox (MPOX) is a zoonotic disease endemic to regions of Central/Western Africa. The geographic endemicity of MPV has expanded, broadening the human-monkeypox virus interface and its potential for spillover. Since May 2022, a large multi-country MPV outbreak with no proven links to endemic countries has originated in Europe and has rapidly expanded around the globe, setting off genomic surveillance efforts. Here, we conducted a genomic analysis of 23 MPV-infected patients from New York City during the early outbreak, assessing the phylogenetic relationship of these strains against publicly available MPV genomes. Additionally, we compared the genomic sequences of clinical isolates versus culture-passaged samples from a subset of samples. Phylogenetic analysis revealed that MPV genomes included in this study cluster within the B.1 lineage (Clade IIb), with some of the samples displaying further differentiation into five different sub-lineages of B.1. Mutational analysis revealed 55 non-synonymous polymorphisms throughout the genome, with some of these mutations located in critical regions required for viral multiplication, structural and assembly functions, as well as the target region for antiviral treatment. In addition, we identified a large majority of polymorphisms associated with GA > AA and TC > TT nucleotide replacements, suggesting the action of human APOBEC3 enzyme. A comparison between clinical isolates and cell culture-passaged samples failed to reveal any difference. Our results provide a first glance at the mutational landscape of early MPV-2022 (B.1) circulating strains in NYC.
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U2 - 10.1080/22221751.2023.2192830
DO - 10.1080/22221751.2023.2192830
M3 - Research Article
C2 - 36927408
AN - SCOPUS:85152637211
SN - 2222-1751
VL - 12
JO - Emerging Microbes and Infections
JF - Emerging Microbes and Infections
IS - 1
M1 - e2192830
ER -
