TY - GEN
T1 - Modification of Polymeric Scaffolds Made of PEG and Nanohydroxyapatite Embedded with Carbon Dots for Applications in Bone Tissue Regeneration
AU - Morales, S. Degiovanni
AU - Pineda, J. S.Ballestas
AU - Daza, M. A.Molina
AU - Celis, P. Mancipe
AU - Ondo-Mendez, A.
AU - Rodríguez Burbano, D. C.
AU - Múnera, M.
N1 - Publisher Copyright:
© 2024, The Author(s), under exclusive license to Springer Nature Switzerland AG.
PY - 2023/12/17
Y1 - 2023/12/17
N2 - Composite scaffolds have been used to treat bone defects from severe trauma, pathologies, or congenital malformations. Polymers such as polyethylene glycol (PEG) and ceramics such as nanohydroxyapatite (nHA) have been used for scaffold fabrication due to their mechanical and biological properties, which favor osteoconduction as well as mimic physical and structural characteristics of natural bone. Nanomaterials, such as carbon dots, have been implemented for this application due to their biocompatible nature and ability to improve mechanical properties and enhance biological interactions. Hydroxyapatite nanoparticles embedded with carbon dots (nHA-CD) were obtained by a rapid continuous precipitation method. Carbon dots (CDs) were synthesized by the microwave-assisted method. Characterization performed by UV-Vis spectrophotometry, Scanning electron microscopy (SEM), and Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) confirmed the incorporation of CDs in nHA. A scaffold based on PEG and nHA embedded with carbon dots (PEG-nHA-CD) was synthesized using a direct mixing method. The scaffold presented a density of 0,48 g/cm3, similar to that of natural bone, indicating similar physical and structural characteristics. The cytotoxicity of the synthesized samples was evaluated by MTT and Trypan Blue assays. The results showed the potential of the PEG-nHA-CD scaffold fabricated for applications in tissue engineering aimed at future bone tissue applications.
AB - Composite scaffolds have been used to treat bone defects from severe trauma, pathologies, or congenital malformations. Polymers such as polyethylene glycol (PEG) and ceramics such as nanohydroxyapatite (nHA) have been used for scaffold fabrication due to their mechanical and biological properties, which favor osteoconduction as well as mimic physical and structural characteristics of natural bone. Nanomaterials, such as carbon dots, have been implemented for this application due to their biocompatible nature and ability to improve mechanical properties and enhance biological interactions. Hydroxyapatite nanoparticles embedded with carbon dots (nHA-CD) were obtained by a rapid continuous precipitation method. Carbon dots (CDs) were synthesized by the microwave-assisted method. Characterization performed by UV-Vis spectrophotometry, Scanning electron microscopy (SEM), and Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) confirmed the incorporation of CDs in nHA. A scaffold based on PEG and nHA embedded with carbon dots (PEG-nHA-CD) was synthesized using a direct mixing method. The scaffold presented a density of 0,48 g/cm3, similar to that of natural bone, indicating similar physical and structural characteristics. The cytotoxicity of the synthesized samples was evaluated by MTT and Trypan Blue assays. The results showed the potential of the PEG-nHA-CD scaffold fabricated for applications in tissue engineering aimed at future bone tissue applications.
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U2 - 10.1007/978-3-031-49401-7_40
DO - 10.1007/978-3-031-49401-7_40
M3 - Conference contribution
AN - SCOPUS:85180632504
SN - 978-3-031-49400-0
VL - 98
T3 - IFMBE Proceedings
SP - 396
EP - 404
BT - 9th Latin American Congress on Biomedical Engineering and 28th Brazilian Congress on Biomedical Engineering - Proceedings of CLAIB and CBEB 2022—Volume 1
A2 - Marques, Jefferson Luiz Brum
A2 - Rodrigues, Cesar Ramos
A2 - Suzuki, Daniela Ota Hisayasu
A2 - García Ojeda, Renato
A2 - Marino Neto, José
PB - Springer Science and Business Media Deutschland GmbH
T2 - 9th Latin American Congress on Biomedical Engineering, CLAIB 2022 and 28th Brazilian Congress on Biomedical Engineering, CBEB 2022
Y2 - 24 October 2022 through 28 October 2022
ER -
