Modification of Polymeric Scaffolds Made of PEG and Nanohydroxyapatite Embedded with Carbon Dots for Applications in Bone Tissue Regeneration

S. Degiovanni Morales, J. S.Ballestas Pineda, M. A.Molina Daza, P. Mancipe Celis, A. Ondo-Mendez, D. C. Rodríguez Burbano, M. Múnera

Research output: Contribution to conferenceConference proceedingspeer-review

Abstract

Composite scaffolds have been used to treat bone defects from severe trauma, pathologies, or congenital malformations. Polymers such as polyethylene glycol (PEG) and ceramics such as nanohydroxyapatite (nHA) have been used for scaffold fabrication due to their mechanical and biological properties, which favor osteoconduction as well as mimic physical and structural characteristics of natural bone. Nanomaterials, such as carbon dots, have been implemented for this application due to their biocompatible nature and ability to improve mechanical properties and enhance biological interactions. Hydroxyapatite nanoparticles embedded with carbon dots (nHA-CD) were obtained by a rapid continuous precipitation method. Carbon dots (CDs) were synthesized by the microwave-assisted method. Characterization performed by UV-Vis spectrophotometry, Scanning electron microscopy (SEM), and Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) confirmed the incorporation of CDs in nHA. A scaffold based on PEG and nHA embedded with carbon dots (PEG-nHA-CD) was synthesized using a direct mixing method. The scaffold presented a density of 0,48 g/cm3, similar to that of natural bone, indicating similar physical and structural characteristics. The cytotoxicity of the synthesized samples was evaluated by MTT and Trypan Blue assays. The results showed the potential of the PEG-nHA-CD scaffold fabricated for applications in tissue engineering aimed at future bone tissue applications.

Translated title of the contributionModificación de andamiajes poliméricos de PEG y nanohidroxiapatita incrustados con puntos de carbono para su aplicación en la regeneración de tejido óseo
Original languageEnglish (US)
Pages396-404
Number of pages9
DOIs
StatePublished - Dec 17 2024
Event9th Latin American Congress on Biomedical Engineering, CLAIB 2022 and 28th Brazilian Congress on Biomedical Engineering, CBEB 2022 - Florianópolis, Brazil
Duration: Oct 24 2022Oct 28 2022

Conference

Conference9th Latin American Congress on Biomedical Engineering, CLAIB 2022 and 28th Brazilian Congress on Biomedical Engineering, CBEB 2022
Country/TerritoryBrazil
CityFlorianópolis
Period10/24/2210/28/22

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

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