Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells

Rocio Tirado; Arturo Ortega; Rosa Elena Sarmiento; Beatríz Gómez

doi:10.1016/j.cellimm.2005.04.003

Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells

Rocio Tirado, Arturo Ortega, Rosa Elena Sarmiento, Beatríz Gómez

Research output: Contribution to journal › Research Article › peer-review

13 Scopus citations

Abstract

The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.

Original language	English (US)
Pages (from-to)	61-71
Number of pages	11
Journal	Cellular Immunology
Volume	233
Issue number	1
DOIs	https://doi.org/10.1016/j.cellimm.2005.04.003
State	Published - Jan 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Immunology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.cellimm.2005.04.003

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@article{c3e65aa6f8cb400890c6cee9636142af,

title = "Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells",

abstract = "The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.",

author = "Rocio Tirado and Arturo Ortega and Sarmiento, {Rosa Elena} and Beatr{\'i}z G{\'o}mez",

note = "Funding Information: We are grateful to Dr. Rafael Bojalil and Dr. Felipe Vadillo for help with IL-1α determinations. We also thank Dr. Gustavo Pedraza-Alva for help with FACS analysis and Dr. Yvonne Rosenstein for her constructive discussions. This investigation was partially supported by grants from Consejo Nacional de Ciencia y Tecnolog{\'i}a (42867/A-1) and from the Direcci{\'o}n General de Asuntos del Personal Acad{\'e}mico (IN203303, IX241804-1). R.T.M. was supported by Programa de Apoyo a Estudiantes de Posgrado (Scholarship 102373) and Sistema Nacional de Investigadores del Consejo Nacional de Ciencia y Tecnolog{\'i}a (Scholarship 21393). The authors thank Dr. Juan Nu{\~n}ez for help with statistical analysis and Veronica Yakoleff for editing the manuscript.",

year = "2005",

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doi = "10.1016/j.cellimm.2005.04.003",

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T1 - Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells

AU - Tirado, Rocio

AU - Ortega, Arturo

AU - Sarmiento, Rosa Elena

AU - Gómez, Beatríz

N1 - Funding Information: We are grateful to Dr. Rafael Bojalil and Dr. Felipe Vadillo for help with IL-1α determinations. We also thank Dr. Gustavo Pedraza-Alva for help with FACS analysis and Dr. Yvonne Rosenstein for her constructive discussions. This investigation was partially supported by grants from Consejo Nacional de Ciencia y Tecnología (42867/A-1) and from the Dirección General de Asuntos del Personal Académico (IN203303, IX241804-1). R.T.M. was supported by Programa de Apoyo a Estudiantes de Posgrado (Scholarship 102373) and Sistema Nacional de Investigadores del Consejo Nacional de Ciencia y Tecnología (Scholarship 21393). The authors thank Dr. Juan Nuñez for help with statistical analysis and Veronica Yakoleff for editing the manuscript.

PY - 2005/1

Y1 - 2005/1

N2 - The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.

AB - The aim of this study was to investigate whether respiratory syncytial virus persistence regulates interleukin 8 (IL-8) mRNA synthesis and protein secretion in a human lung epithelial cell line (A549). Therefore, we established RSV persistence in these cells (A549per) and determined the levels of interleukin-8 mRNA by RT-PCR and of protein through ELISA. Interleukin-8 mRNA synthesis and protein secretion were continuously up-regulated in A549per cells during passages and in A549 cells that had been incubated with supernatants (cA549per) obtained from A549per passages. These results suggested that the enhancement of interleukin-8 was stimulated either by the presence of the RSV genome in the cell or by soluble mediator(s) induced by RSV, which, in turn, increased interleukin-8 mRNA synthesis and protein secretion. Soluble RSV F and G proteins were identified as mediators. Moreover, interleukin-8 enhancement was observed after 1-min incubation with the soluble mediators, thus suggesting that interleukin-8 up-regulation was triggered by receptor-ligand interaction.

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Interleukin-8 mRNA synthesis and protein secretion are continuously up-regulated by respiratory syncytial virus persistently infected cells

Abstract

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