Glucosylceramides from Lomentospora prolificans induce a differential production of cytokines and increases the microbicidal activity of macrophages.

Mariana Xisto, Julian Esteban Munoz Henao, Lucas Días, Giulia Maria Pires Santos, Renata de Oliveira Rocha Calixto, Mariana Collodetti Bernardino, Carlos Taborda, Eliana Barreto-Bergter

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4 Scopus citations

Abstract

Lomentospora prolificans is an emerging opportunistic fungus with a high resistance to antifungal agents and it can cause localized infections in immunocompetent patients and disseminated infections with a high mortality rate in immunosuppressed patients. Glucosylceramides (GlcCer) are synthetized in the majority of known fungal pathogens. They are bioactive molecules presenting different functions, such as involvement in fungal growth and morphological transitions in several fungi. The elucidation of the primary structure of the fungal surface glycoconjugates could contribute for the understanding of the mechanisms of pathogenicity. In this work, GlcCer species were isolated from mycelium and conidia forms of L. prolificans and their chemical structures were elucidated by mass spectrometry (ESI-MS). GlcCer purified from both forms presented a major species at m/z 750 that corresponds to N-2-hydroxyhexadecanoyl-1-β-D-glucopyranosyl-9-methyl-4,8-sphingadienine. Monoclonal antibodies against GlcCer could recognize L. prolificans GlcCer species from mycelium and conidia, suggesting a conserved epitope in fungal GlcCer. In addition, in vivo assays showed that purified GlcCer species from both forms was able to induce a high secretion of pro-inflammatory cytokines by splenocytes. GlcCer species also promote the recruitment of polymorphonuclear, eosinophils, small peritoneal macrophage (SPM) and mononuclear cells to the peritoneal cavity. GlcCer species were also able to induce the oxidative burst by peritoneal macrophages with NO and superoxide radicals production, and to increase the killing of L. prolificans conidia by peritoneal macrophages. These results indicate that GlcCer species from L. prolificans are a potent immune response activator.
Translated title of the contributionLas glucosilceramidas de Lomentospora prolificans inducen una producción diferencial de citocinas y aumentan la actividad microbicida de los macrófagos.
Original languageEnglish
Article number554
Pages (from-to)1
Number of pages13
JournalFrontiers in Microbiology
Volume10
Issue numberMAR
DOIs
StatePublished - Mar 22 2019

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Microbiology(all)

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