TY - JOUR
T1 - Genetic diversity of puerto rican farmer-held papaya (Carica papaya) using SSR markers
AU - Luciano-Rosario, Dianiris
AU - Cruz-Saavedra, Luis A.
AU - Siritunga, Dimuth
N1 - Funding Information:
Received for publication 31 Jan. 2018. Accepted for publication 31 May 2018. We thank David Jenkins (USDA-Tropical Agricultural Research station, Mayaguez, PR) for the acquisition of the USDA germplasm and some of the commercial samples, and Timothy Porch (USDA-Tropical Agricultural Research station, Mayaguez, PR) for the provision of equipment necessary to complete this project. This project was supported by a grant to DS from the Howard Hughes Medical Institute (award number 52007566) and USDA HIS program (2016-06469). 1Corresponding author. E-mail: dimuth.siritunga@ upr.edu.
Publisher Copyright:
© 2018, American Society for Horticultural Science. All rights reserved.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2018/8
Y1 - 2018/8
N2 - Native to Central America, papaya (Carica papaya) is one of the most cultivated fruit crops in the tropical areas of the world. Genetic diversity analyses are an important aspect of conservation of plant genetic resources. In the island of Puerto Rico, where papaya has been consumed for centuries, knowledge on the genetic diversity of papaya is lacking. Therefore, 162 papaya accessions were evaluated using 23 simple sequence repeat (SSR) markers. Of these accessions, 139 were farmer-held samples from Puerto Rico, 13 were U.S. Department of Agriculture (USDA) repository samples, and 10 were commercial varieties. A total of 214 alleles were identified with a mean observed heterozygosity (Ho) of 0.219. Inbreeding coefficient (F) was 0.565, and when evaluating the population structure of these accessions, 2 groups (k = 2) were identified. Unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed no geographical organization within the unknown Puerto Rican samples. This assessment provides an extensive record of the genetic diversity of papaya in Puerto Rico which can contribute to breeding strategies and to the conservation of papaya genetic resources in the Caribbean.
AB - Native to Central America, papaya (Carica papaya) is one of the most cultivated fruit crops in the tropical areas of the world. Genetic diversity analyses are an important aspect of conservation of plant genetic resources. In the island of Puerto Rico, where papaya has been consumed for centuries, knowledge on the genetic diversity of papaya is lacking. Therefore, 162 papaya accessions were evaluated using 23 simple sequence repeat (SSR) markers. Of these accessions, 139 were farmer-held samples from Puerto Rico, 13 were U.S. Department of Agriculture (USDA) repository samples, and 10 were commercial varieties. A total of 214 alleles were identified with a mean observed heterozygosity (Ho) of 0.219. Inbreeding coefficient (F) was 0.565, and when evaluating the population structure of these accessions, 2 groups (k = 2) were identified. Unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed no geographical organization within the unknown Puerto Rican samples. This assessment provides an extensive record of the genetic diversity of papaya in Puerto Rico which can contribute to breeding strategies and to the conservation of papaya genetic resources in the Caribbean.
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U2 - 10.21273/HORTSCI12943-18
DO - 10.21273/HORTSCI12943-18
M3 - Research Article
AN - SCOPUS:85052494237
SN - 0018-5345
VL - 53
SP - 1109
EP - 1114
JO - HortScience
JF - HortScience
IS - 8
ER -
