TY - JOUR
T1 - Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein
AU - Patarroyo, Manuel A.
AU - Plaza, David F.
AU - Ocampo, Marisol
AU - Curtidor, Hernando
AU - Forero, Martha
AU - Rodriguez, Luis E.
AU - Patarroyo, Manuel E.
N1 - Funding Information:
This research has been supported by COLCIENCIAS contract RC-2008. Nora Martinez translated this manuscript.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/8/8
Y1 - 2008/8/8
N2 - Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine.
AB - Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine.
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U2 - 10.1016/j.bbrc.2008.05.157
DO - 10.1016/j.bbrc.2008.05.157
M3 - Article
C2 - 18539140
AN - SCOPUS:45449101647
SN - 0006-291X
VL - 372
SP - 935
EP - 940
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
