Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein

Manuel A. Patarroyo, David F. Plaza, Marisol Ocampo, Hernando Curtidor, Martha Forero, Luis E. Rodriguez, Manuel E. Patarroyo

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine.

Original languageEnglish (US)
Pages (from-to)935-940
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume372
Issue number4
DOIs
StatePublished - Aug 8 2008

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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