TY - JOUR
T1 - A polymorphism in the inducible nitric oxide synthase gene is associated with tuberculosis
AU - Miguel Gómez, Luis
AU - Anaya, Juan Manuel
AU - Ramón Vilchez, José
AU - Cadena, Jose
AU - Hinojosa, Rosa
AU - Vélez, Luciano
AU - Angel Lopez-Nevot, Miguel
AU - Martín, Javier
N1 - Funding Information:
This work has been supported by Grant SAF03-3460 from Plan Nacional de I+D, and in part by the Junta de Andalucía, grupo CTS-180. We would like to thank all patients and participants of this study. LMG's work was financed in part by an educational grant from Misión Social-TCC, Colombia.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2007/7
Y1 - 2007/7
N2 - iNOS or NOS2 is a molecule that plays a key role in the immunological control of a broad spectrum of infectious agents. Investigation is hampered by difficulty in estimating in vivo production of nitric oxide (NO), but genetic studies provide a potential means of examining the relation between NO production and disease outcome. To better characterize the host genetic factors determining the susceptibility to TB, we evaluated the influence of two polymorphisms in the NOS2A gene on the risk of developing pulmonary TB in a Northwestern Colombian population, which is a moderately-high endemic area. One hundred and fourteen patients with TB and negative for human immunodeficiency virus, plus 304 healthy controls were examined for NOS2A CCTTT and TAAA polymorphisms. A total of 160 healthy controls mentioned before, underwent tuberculin skin test (TST). Analysis disclosed significant differences between patients and controls with NOS2A CCTTT polymorphism (P=0.0001, Pc=0.001, OR=0.4, and 95%CI=0.3-0.7) independent of TST status. When the NOS2A alleles were stratified into short (8-11) and long (12-16) repeats, significant differences with short repeats were observed between TB patients and all controls (P=0.005, OR=0.63, 95%CI=0.46-0.86). No individual association with NOS2A TAAA was detected. These results indicate that a polymorphism in the NOS2A gene influences the susceptibility to TB and suggest a role for NOS2A in the pathogenesis of mycobacterial infection.
AB - iNOS or NOS2 is a molecule that plays a key role in the immunological control of a broad spectrum of infectious agents. Investigation is hampered by difficulty in estimating in vivo production of nitric oxide (NO), but genetic studies provide a potential means of examining the relation between NO production and disease outcome. To better characterize the host genetic factors determining the susceptibility to TB, we evaluated the influence of two polymorphisms in the NOS2A gene on the risk of developing pulmonary TB in a Northwestern Colombian population, which is a moderately-high endemic area. One hundred and fourteen patients with TB and negative for human immunodeficiency virus, plus 304 healthy controls were examined for NOS2A CCTTT and TAAA polymorphisms. A total of 160 healthy controls mentioned before, underwent tuberculin skin test (TST). Analysis disclosed significant differences between patients and controls with NOS2A CCTTT polymorphism (P=0.0001, Pc=0.001, OR=0.4, and 95%CI=0.3-0.7) independent of TST status. When the NOS2A alleles were stratified into short (8-11) and long (12-16) repeats, significant differences with short repeats were observed between TB patients and all controls (P=0.005, OR=0.63, 95%CI=0.46-0.86). No individual association with NOS2A TAAA was detected. These results indicate that a polymorphism in the NOS2A gene influences the susceptibility to TB and suggest a role for NOS2A in the pathogenesis of mycobacterial infection.
UR - http://www.scopus.com/inward/record.url?scp=34250656205&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250656205&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2007.03.002
DO - 10.1016/j.tube.2007.03.002
M3 - Article
C2 - 17475563
AN - SCOPUS:34250656205
SN - 1472-9792
VL - 87
SP - 288
EP - 294
JO - Tuberculosis
JF - Tuberculosis
IS - 4
ER -
