A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents

Luciano Polonelli; Tecla Ciociola; Lisa Elviri; Pier Paolo Zanello; Laura Giovati; Denise C. Arruda; Julián E. Muñoz; Renato A. Mortara; Giulia Morace; Elisa Borghi; Serena Galati; Oriano Marin; Claudio Casoli; Elisabetta Pilotti; Paola Ronzi; Luiz R. Travassos; Walter Magliani; Stefania Conti

doi:10.1038/srep35018

A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents

Luciano Polonelli, Tecla Ciociola, Lisa Elviri, Pier Paolo Zanello, Laura Giovati, Denise C. Arruda, Julián E. Muñoz, Renato A. Mortara, Giulia Morace, Elisa Borghi, Serena Galati, Oriano Marin, Claudio Casoli, Elisabetta Pilotti, Paola Ronzi, Luiz R. Travassos, Walter Magliani, Stefania Conti

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multifaceted functionality of humoral immunity.

Original language	English (US)
Article number	35018
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep35018
State	Published - Oct 11 2016
Externally published	Yes

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Polonelli, L., Ciociola, T., Elviri, L., Zanello, P. P., Giovati, L., Arruda, D. C., Muñoz, J. E., Mortara, R. A., Morace, G., Borghi, E., Galati, S., Marin, O., Casoli, C., Pilotti, E., Ronzi, P., Travassos, L. R., Magliani, W., & Conti, S. (2016). A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents. Scientific Reports, 6, Article 35018. https://doi.org/10.1038/srep35018

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title = "A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents",

abstract = "A phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multifaceted functionality of humoral immunity.",

author = "Luciano Polonelli and Tecla Ciociola and Lisa Elviri and Zanello, {Pier Paolo} and Laura Giovati and Arruda, {Denise C.} and Mu{\~n}oz, {Juli{\'a}n E.} and Mortara, {Renato A.} and Giulia Morace and Elisa Borghi and Serena Galati and Oriano Marin and Claudio Casoli and Elisabetta Pilotti and Paola Ronzi and Travassos, {Luiz R.} and Walter Magliani and Stefania Conti",

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doi = "10.1038/srep35018",

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Polonelli, L, Ciociola, T, Elviri, L, Zanello, PP, Giovati, L, Arruda, DC, Muñoz, JE, Mortara, RA, Morace, G, Borghi, E, Galati, S, Marin, O, Casoli, C, Pilotti, E, Ronzi, P, Travassos, LR, Magliani, W & Conti, S 2016, 'A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents', Scientific Reports, vol. 6, 35018. https://doi.org/10.1038/srep35018

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T1 - A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents

AU - Polonelli, Luciano

AU - Ciociola, Tecla

AU - Elviri, Lisa

AU - Zanello, Pier Paolo

AU - Giovati, Laura

AU - Arruda, Denise C.

AU - Muñoz, Julián E.

AU - Mortara, Renato A.

AU - Morace, Giulia

AU - Borghi, Elisa

AU - Galati, Serena

AU - Marin, Oriano

AU - Casoli, Claudio

AU - Pilotti, Elisabetta

AU - Ronzi, Paola

AU - Travassos, Luiz R.

AU - Magliani, Walter

AU - Conti, Stefania

PY - 2016/10/11

Y1 - 2016/10/11

N2 - A phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multifaceted functionality of humoral immunity.

AB - A phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multifaceted functionality of humoral immunity.

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A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents

Abstract

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