Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

Elizabeth Torres-Morales, Laura Taborda, Nestor Cardona, Alejandra De-la-Torre, Juan Carlos Sepulveda-Arias, Manuel Alfonso Patarroyo, Jorge Enrique Gomez-Marin

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

28 Citas (Scopus)

Resumen

We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.

Idioma originalInglés estadounidense
Páginas (desde-hasta)315-322
Número de páginas8
PublicaciónMedical Microbiology and Immunology
Volumen203
N.º5
DOI
EstadoPublicada - sep 26 2014

All Science Journal Classification (ASJC) codes

  • Inmulogía y alergología
  • Inmunología
  • Microbiología (médica)

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