TY - JOUR
T1 - Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis
AU - Torres-Morales, Elizabeth
AU - Taborda, Laura
AU - Cardona, Nestor
AU - De-la-Torre, Alejandra
AU - Sepulveda-Arias, Juan Carlos
AU - Patarroyo, Manuel Alfonso
AU - Gomez-Marin, Jorge Enrique
N1 - Funding Information:
Acknowledgments Financed by grants 111351929258 from Col-ciencias (Colombian Scientific Government Agency) and 5-09-3 from the Universidad Tecnológica de Pereira.
Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
PY - 2014/9/26
Y1 - 2014/9/26
N2 - We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.
AB - We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.
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U2 - 10.1007/s00430-014-0339-0
DO - 10.1007/s00430-014-0339-0
M3 - Article
C2 - 24816543
AN - SCOPUS:84919477257
SN - 0300-8584
VL - 203
SP - 315
EP - 322
JO - Medical Microbiology and Immunology
JF - Medical Microbiology and Immunology
IS - 5
ER -