Susceptibility of Campylobacter jejuni to organic acids and monoacylglycerols

Z. Molatová, E. Skřivanová, B. Macias, N. R. Mcewan, P. Březina, M. Marounek

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

22 Citas (Scopus)

Resumen

Organic acids can be used as feed supplements or for treatment of poultry carcasses in processing plants. The antimicrobial activity of nineteen organic acids and two monoacylglycerols in cultures of Campylobacter jejuni CCM 6214T (ATCC 33560) was determined using a SYBR Green-based real-time PCR assay. The IC50 was a concentration at which only 50 % of a bacteria specific DNA sequence was amplified. Caprylic, capric and lauric acids were the most efficient antimicrobials among the compounds tested (IC50 ≤ 0.1 mg/mL). In a weakly acidic environment (pH 5.5), the antimicrobial activity was more pronounced than at pH 6.5. At pH 5.5, oleic and fumaric acid also had clear antimicrobial activity, as did monocaprylin. The antimicrobial activity of acetic, butyric, stearic and succinic acid was low. In cells treated with fumaric acid, the potential of potassium and tetraphenylphosphonium ion-selective electrodes changed, indicating an increase in cytoplasmic and outer membrane permeability, respectively. No changes in membrane permeability were observed in cells treated with capric acid or monocaprin. Transmission electron microscopy revealed separation of the inner and outer membrane in cells treated with capric and fumaric acid, as well as cytoplasmic disorganization in cells exposed to capric acid.

Idioma originalInglés estadounidense
Páginas (desde-hasta)215-220
Número de páginas6
PublicaciónFolia Microbiologica
Volumen55
N.º3
DOI
EstadoPublicada - 2010
Publicado de forma externa

All Science Journal Classification (ASJC) codes

  • Microbiología

Huella Profundice en los temas de investigación de 'Susceptibility of Campylobacter jejuni to organic acids and monoacylglycerols'. En conjunto forman una huella única.

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