TY - JOUR
T1 - Spontaneous shift of an hsv type 1 productive, persistently infected macrophage-like culture to a non-productive culture
AU - Tirado, R.
AU - Sarmiento, R. E.
AU - Gómez, B.
N1 - Funding Information:
This work was partially supported by grants Universi-dad Nacional Aut6noma de M6xico, IN 200894, and 2211 PUIS.
PY - 1998
Y1 - 1998
N2 - A herpes simplex virus type 1 (HSV1) productive, persistently infected murine macrophage-like culture was obtained by infection at low multiplicity (0.001). Persistent cultures were characterized by spontaneously ceasing to produce infectious viruses after initial HSV1 production. A similar pattern of virus production was observed in cultures obtained by reinfection of the persistent macrophages with the original virus. Nonetheless, in persistent and reinfected cultures (17/18) which did not produce infectious viruses, vital proteins were detected by immunoblot, although their molecular weight, number and pattern differed among the cultures.
AB - A herpes simplex virus type 1 (HSV1) productive, persistently infected murine macrophage-like culture was obtained by infection at low multiplicity (0.001). Persistent cultures were characterized by spontaneously ceasing to produce infectious viruses after initial HSV1 production. A similar pattern of virus production was observed in cultures obtained by reinfection of the persistent macrophages with the original virus. Nonetheless, in persistent and reinfected cultures (17/18) which did not produce infectious viruses, vital proteins were detected by immunoblot, although their molecular weight, number and pattern differed among the cultures.
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U2 - 10.1016/S0923-2516(98)80032-0
DO - 10.1016/S0923-2516(98)80032-0
M3 - Article
C2 - 9711538
AN - SCOPUS:0032079139
SN - 0923-2516
VL - 149
SP - 145
EP - 151
JO - Research in Virology
JF - Research in Virology
IS - 3
ER -