Selecting barcoding loci for plants: Evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants

Michelle L. Hollingsworth, Alex Andra Clark, Laura L. Forrest, James Richardson, R. Toby Pennington, David G. Long, Robyn Cowan, Mark W. Chase, Myriam Gaudeul, Peter M. Hollingsworth

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

307 Citas (Scopus)

Resumen

There has been considerable debate, but little consensus regarding locus choice for DNA barcoding land plants. This is partly attributable to a shortage of comparable data from all proposed candidate loci on a common set of samples. In this study, we evaluated the seven main candidate plastid regions (rpoC1, rpoB, rbcL, matK, trnH-psbA, atpF-atpH, psbK-psbI) in three divergent groups of land plants [Inga (angiosperm); Araucaria (gymnosperm); Asterella s.l. (liverwort)]. Across these groups, no single locus showed high levels of universality and resolvability. Interspecific sharing of sequences from individual loci was common. However, when multiple loci were combined, fewer barcodes were shared among species. Evaluation of the performance of previously published suggestions of particular multilocus barcode combinations showed broadly equivalent performance. Minor improvements on these were obtained by various new three-locus combinations involving rpoC1, rbcL, matK and trnH-psbA, but no single combination clearly outperformed all others. In terms of absolute discriminatory power, promising results occurred in liverworts (e.g. c. 90% species discrimination based on rbcL alone). However, Inga (rapid radiation) and Araucaria (slow rates of substitution) represent challenging groups for DNA barcoding, and their corresponding levels of species discrimination reflect this (upper estimate of species discrimination = 69% in Inga and only 32% in Araucaria; mean = 60% averaging all three groups).

Idioma originalInglés estadounidense
Páginas (desde-hasta)439-457
Número de páginas19
PublicaciónMolecular Ecology Resources
Volumen9
N.º2
DOI
EstadoPublicada - mar. 2009
Publicado de forma externa

Áreas temáticas de ASJC Scopus

  • Biotecnología
  • Ecología, evolución, comportamiento y sistemática
  • Genética

Huella

Profundice en los temas de investigación de 'Selecting barcoding loci for plants: Evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants'. En conjunto forman una huella única.

Citar esto