Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

M. Cogliati; P. E. Chidebelu; M. Hitchcock; M. Chen; V. Rickerts; S. Ackermann; M. Desnos Ollivier; J. Inácio; U. Nawrot; M. Florek; K. J. Kwon-Chung; D. H. Yang; C. Firacative; C. A. Puime; P. Escandon; S. Bertout; F. Roger; J. Xu

doi:10.1016/j.fgb.2023.103861

Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

Título traducido de la contribución: Tipificación de secuencias multilocus y filogenética de híbridos AD de Cryptococcus neoformans

M. Cogliati, P. E. Chidebelu, M. Hitchcock, M. Chen, V. Rickerts, S. Ackermann, M. Desnos Ollivier, J. Inácio, U. Nawrot, M. Florek, K. J. Kwon-Chung, D. H. Yang, C. Firacative, C. A. Puime, P. Escandon, S. Bertout, F. Roger, J. Xu

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Resumen

En muchas partes del mundo se han descrito cepas AD híbridas del complejo de especies Cryptococcus neoformans, patógeno para el ser humano. Sin embargo, su origen, diversidad y evolución no se conocen del todo. En este estudio analizamos 102 cepas híbridas de AD procedentes de 21 países de los cinco continentes. Para cada cepa, obtuvimos su tipo de apareamiento y sus secuencias alélicas en cada uno de los siete loci que han sido utilizados para el genotipado de cepas haploides de los serotipos A y D del complejo de especies por la comunidad investigadora de Cryptococcus. Nuestros resultados mostraron que la mayoría de los híbridos AD presentaban pérdida de heterocigosidad en uno o más de los siete loci analizados. Los análisis filogenéticos y genéticos poblacionales de las secuencias alélicas revelaron múltiples orígenes de los híbridos dentro de cada continente, remontándose a hace un millón de años en África y hasta el presente en otros continentes. Encontramos pruebas de reproducción clonal y dispersión a larga distancia de estos híbridos en la naturaleza. Las comparaciones con las cepas haploides globales de los serotipos A y D identificaron nuevos alelos y nuevos genotipos haploides multilocus en los híbridos AD, lo que concuerda con la presencia de una diversidad genética aún por descubrir en las poblaciones haploides de este complejo de especies en la naturaleza. En conjunto, nuestros resultados indican que los híbridos AD pueden genotiparse eficazmente utilizando el mismo enfoque de secuenciación multilocus que el establecido para las cepas de los serotipos A y D. Nuestras comparaciones de los híbridos AD entre sí, así como con las cepas haploides globales de los serotipos A y D, revelaron una nueva diversidad genética, así como pruebas de múltiples orígenes y de la evolución dinámica de estos híbridos en la naturaleza.

Título traducido de la contribución	Tipificación de secuencias multilocus y filogenética de híbridos AD de Cryptococcus neoformans
Idioma original	Inglés estadounidense
Número de artículo	103861
Páginas (desde-hasta)	1-13
Número de páginas	13
Publicación	Fungal Genetics and Biology
Volumen	170
DOI	https://doi.org/10.1016/j.fgb.2023.103861
Estado	Publicación electrónica previa a su impresión - dic. 19 2023

Áreas temáticas de ASJC Scopus

Microbiología
Genética

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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Cogliati, M., Chidebelu, P. E., Hitchcock, M., Chen, M., Rickerts, V., Ackermann, S., Desnos Ollivier, M., Inácio, J., Nawrot, U., Florek, M., Kwon-Chung, K. J., Yang, D. H., Firacative, C., Puime, C. A., Escandon, P., Bertout, S., Roger, F., & Xu, J. (2023). Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids. Fungal Genetics and Biology, 170, 1-13. Artículo 103861. Avanzar publicación online. https://doi.org/10.1016/j.fgb.2023.103861

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title = "Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids",

abstract = "Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.",

author = "M. Cogliati and Chidebelu, {P. E.} and M. Hitchcock and M. Chen and V. Rickerts and S. Ackermann and {Desnos Ollivier}, M. and J. In{\'a}cio and U. Nawrot and M. Florek and Kwon-Chung, {K. J.} and Yang, {D. H.} and C. Firacative and Puime, {C. A.} and P. Escandon and S. Bertout and F. Roger and J. Xu",

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day = "19",

doi = "10.1016/j.fgb.2023.103861",

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Cogliati, M, Chidebelu, PE, Hitchcock, M, Chen, M, Rickerts, V, Ackermann, S, Desnos Ollivier, M, Inácio, J, Nawrot, U, Florek, M, Kwon-Chung, KJ, Yang, DH, Firacative, C, Puime, CA, Escandon, P, Bertout, S, Roger, F & Xu, J 2023, 'Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids', Fungal Genetics and Biology, vol. 170, 103861, pp. 1-13. https://doi.org/10.1016/j.fgb.2023.103861

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T1 - Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

AU - Cogliati, M.

AU - Chidebelu, P. E.

AU - Hitchcock, M.

AU - Chen, M.

AU - Rickerts, V.

AU - Ackermann, S.

AU - Desnos Ollivier, M.

AU - Inácio, J.

AU - Nawrot, U.

AU - Florek, M.

AU - Kwon-Chung, K. J.

AU - Yang, D. H.

AU - Firacative, C.

AU - Puime, C. A.

AU - Escandon, P.

AU - Bertout, S.

AU - Roger, F.

AU - Xu, J.

PY - 2023/12/19

Y1 - 2023/12/19

N2 - Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.

AB - Hybrid AD strains of the human pathogenic Cryptococcus neoformans species complex have been reported from many parts of the world. However, their origin, diversity, and evolution are incompletely understood. In this study, we analyzed 102 AD hybrid strains representing 21 countries on five continents. For each strain, we obtained its mating type and its allelic sequences at each of the seven loci that have been used for genotyping haploid serotypes A and D strains of the species complex by the Cryptococcus research community. Our results showed that most AD hybrids exhibited loss of heterozygosity at one or more of the seven analyzed loci. Phylogenetic and population genetic analyses of the allelic sequences revealed multiple origins of the hybrids within each continent, dating back to one million years ago in Africa and up to the present in other continents. We found evidence for clonal reproduction and long-distance dispersal of these hybrids in nature. Comparisons with the global haploid serotypes A and D strains identified new alleles and new haploid multi-locus genotypes in AD hybrids, consistent with the presence of yet-to-be discovered genetic diversity in haploid populations of this species complex in nature. Together, our results indicate that AD hybrids can be effectively genotyped using the same multi-locus sequencing type approach as that established for serotypes A and D strains. Our comparisons of the AD hybrids among each other as well as with the global haploid serotypes A and D strains revealed novel genetic diversity as well as evidence for multiple origins and dynamic evolution of these hybrids in nature.

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Multi-locus sequence typing and phylogenetics of Cryptococcus neoformans AD hybrids

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