Molecular evidence of Borrelia spp. in bats from Córdoba Department, northwest Colombia

Yesica López; Sebastián Muñoz-Leal; Caty Martínez; Camilo Guzmán; Alfonso Calderón; Jairo Martínez; Ketty Galeano; Marina Muñoz; Juan David Ramírez; Álvaro A. Faccini-Martínez; Salim Mattar

doi:10.1186/s13071-022-05614-y

Molecular evidence of Borrelia spp. in bats from Córdoba Department, northwest Colombia

Título traducido de la contribución: Evidencia molecular de Borrelia spp. en murciélagos del Departamento de Córdoba, noroeste de Colombia

Yesica López, Sebastián Muñoz-Leal, Caty Martínez, Camilo Guzmán, Alfonso Calderón, Jairo Martínez, Ketty Galeano, Marina Muñoz, Juan David Ramírez, Álvaro A. Faccini-Martínez, Salim Mattar

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2 Citas (Scopus)

Resumen

Antecedentes: El género Borrelia está compuesto por dos grupos monofiléticos bien definidos, el complejo Borrelia burgdorferi sensu lato (Bb) y el grupo borreliae de fiebre recurrente (RF). Recientemente se ha descrito un tercer grupo, asociado a reptiles y equidnas. En general, las borreliae del grupo RF utilizan roedores como huéspedes reservorios; aunque los murciélagos neotropicales también pueden estar involucrados como hospedadores importantes, existiendo escaso conocimiento sobre esta asociación. El objetivo de este estudio fue detectar la presencia de Borrelia spp. ADN en murciélagos del departamento de Córdoba en el noroeste de Colombia. Métodos: Durante septiembre de 2020 y junio de 2021 se capturaron 205 murciélagos en seis municipios del departamento de Córdoba, Colombia. Los especímenes se identificaron mediante claves taxonómicas y se extrajo ADN de muestras de bazo. Se realizó una PCR en tiempo real específica de Borrelia para el gen 16S rRNA. Se amplificaron fragmentos de los genes 16S rRNA y flaB en las muestras positivas mediante PCR convencional. Los amplicones detectados fueron secuenciados por el método de Sanger. La reconstrucción filogenética se realizó en IQ-TREE con máxima probabilidad basada en el modelo de sustitución TPM3+F+I+G4 con valores de arranque deducidos de 1000 réplicas. Resultados: En general, el 10,2% (21/205) de las muestras resultaron positivas mediante qPCR; de estos, el 81% (17/21) y el 66,6% (14/21) amplificaron los genes 16S rRNA y flaB, respectivamente. Luego, las muestras positivas para qPCR se sometieron a una PCR anidada y semianidada convencional para amplificar los fragmentos del gen 16S rRNA y flaB. Se secuenciaron nueve muestras positivas para ambos genes, y siete y seis secuencias fueron de buena calidad para los genes 16S rRNA y flaB, respectivamente. El ADN de Borrelia spp. se detectó en los murciélagos insectívoros y frugívoros Artibeus lituratus, Carollia perspicillata, Glossophaga soricina, Phyllostomus discolor y Uroderma sp. Las secuencias del gen 16S rRNA mostraron una identidad del 97,66 al 98,47% con “Borrelia sp. clon Omi3”, “Borrelia sp. RT1S” y Borrelia sp. 2374; las identidades más cercanas para el gen flaB fueron 94,02–98,04% con “Borrelia sp. Macaregua.” Para el gen 16S rRNA, el análisis filogenético mostró un agrupamiento con “Candidatus Borrelia ivorensis” y “Ca. Borrelia africana”, y para el gen flaB mostró una agrupación con Borrelia sp. Macaregua y Borrelia sp. Potiretama. Se desconoce el papel patogénico de la Borrelia detectada en este estudio. Conclusiones: Describimos la primera evidencia molecular de Borrelia spp. en el departamento de Córdoba, Colombia, destacando que varias especies de murciélagos albergan espiroquetas de Borrelia

Título traducido de la contribución	Evidencia molecular de Borrelia spp. en murciélagos del Departamento de Córdoba, noroeste de Colombia
Idioma original	Inglés estadounidense
Número de artículo	5
Publicación	Parasites and Vectors
Volumen	16
N.º	1
DOI	https://doi.org/10.1186/s13071-022-05614-y
Estado	Publicada - dic. 2023

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Parasitología
Veterinaria General
Enfermedades infecciosas

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@article{c28baa6fa08f457986525ab31122d8cc,

title = "Molecular evidence of Borrelia spp. in bats from C{\'o}rdoba Department, northwest Colombia",

abstract = "Background: The genus Borrelia is composed of two well-defined monophyletic groups, the Borrelia burgdorferi sensu lato complex (Bb) and the relapsing fever (RF) group borreliae. Recently, a third group, associated with reptiles and echidnas, has been described. In general, RF group borreliae use rodents as reservoir hosts; although neotropical bats may also be involved as important hosts, with scarce knowledge regarding this association. The objective of this study was to detect the presence of Borrelia spp. DNA in bats from the department of C{\'o}rdoba in northwest Colombia. Methods: During September 2020 and June 2021, 205 bats were captured in six municipalities of C{\'o}rdoba department, Colombia. Specimens were identified using taxonomic keys and DNA was extracted from spleen samples. A Borrelia-specific real-time PCR was performed for the 16S rRNA gene. Fragments of the 16S rRNA and flaB genes were amplified in the positive samples by conventional PCR. The detected amplicons were sequenced by the Sanger method. Phylogenetic reconstruction was performed in IQ-TREE with maximum likelihood based on the substitution model TPM3+F+I+G4 with bootstrap values deduced from 1000 replicates. Results: Overall, 10.2% (21/205) of the samples were found positive by qPCR; of these, 81% (17/21) and 66.6% (14/21) amplified 16S rRNA and flaB genes, respectively. qPCR-positive samples were then subjected to conventional nested and semi-nested PCR to amplify 16S rRNA and flaB gene fragments. Nine positive samples for both genes were sequenced, and seven and six sequences were of good quality for the 16S rRNA and flaB genes, respectively. The DNA of Borrelia spp. was detected in the insectivorous and fruit bats Artibeus lituratus, Carollia perspicillata, Glossophaga soricina, Phyllostomus discolor, and Uroderma sp. The 16S rRNA gene sequences showed 97.66–98.47% identity with “Borrelia sp. clone Omi3,” “Borrelia sp. RT1S,” and Borrelia sp. 2374; the closest identities for the flaB gene were 94.02–98.04% with “Borrelia sp. Macaregua.” For the 16S rRNA gene, the phylogenetic analysis showed a grouping with “Candidatus Borrelia ivorensis” and “Ca. Borrelia africana,” and for the flaB gene showed a grouping with Borrelia sp. Macaregua and Borrelia sp. Potiretama. The pathogenic role of the Borrelia detected in this study is unknown. Conclusions: We describe the first molecular evidence of Borrelia spp. in the department of C{\'o}rdoba, Colombia, highlighting that several bat species harbor Borrelia spirochetes. Graphical Abstract: [Figure not available: see fulltext.].",

author = "Yesica L{\'o}pez and Sebasti{\'a}n Mu{\~n}oz-Leal and Caty Mart{\'i}nez and Camilo Guzm{\'a}n and Alfonso Calder{\'o}n and Jairo Mart{\'i}nez and Ketty Galeano and Marina Mu{\~n}oz and Ram{\'i}rez, {Juan David} and Faccini-Mart{\'i}nez, {{\'A}lvaro A.} and Salim Mattar",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = dec,

doi = "10.1186/s13071-022-05614-y",

language = "English (US)",

volume = "16",

journal = "Parasites and Vectors",

issn = "1756-3305",

publisher = "BioMed Central",

number = "1",

}

TY - JOUR

T1 - Molecular evidence of Borrelia spp. in bats from Córdoba Department, northwest Colombia

AU - López, Yesica

AU - Muñoz-Leal, Sebastián

AU - Martínez, Caty

AU - Guzmán, Camilo

AU - Calderón, Alfonso

AU - Martínez, Jairo

AU - Galeano, Ketty

AU - Muñoz, Marina

AU - Ramírez, Juan David

AU - Faccini-Martínez, Álvaro A.

AU - Mattar, Salim

PY - 2023/12

Y1 - 2023/12

N2 - Background: The genus Borrelia is composed of two well-defined monophyletic groups, the Borrelia burgdorferi sensu lato complex (Bb) and the relapsing fever (RF) group borreliae. Recently, a third group, associated with reptiles and echidnas, has been described. In general, RF group borreliae use rodents as reservoir hosts; although neotropical bats may also be involved as important hosts, with scarce knowledge regarding this association. The objective of this study was to detect the presence of Borrelia spp. DNA in bats from the department of Córdoba in northwest Colombia. Methods: During September 2020 and June 2021, 205 bats were captured in six municipalities of Córdoba department, Colombia. Specimens were identified using taxonomic keys and DNA was extracted from spleen samples. A Borrelia-specific real-time PCR was performed for the 16S rRNA gene. Fragments of the 16S rRNA and flaB genes were amplified in the positive samples by conventional PCR. The detected amplicons were sequenced by the Sanger method. Phylogenetic reconstruction was performed in IQ-TREE with maximum likelihood based on the substitution model TPM3+F+I+G4 with bootstrap values deduced from 1000 replicates. Results: Overall, 10.2% (21/205) of the samples were found positive by qPCR; of these, 81% (17/21) and 66.6% (14/21) amplified 16S rRNA and flaB genes, respectively. qPCR-positive samples were then subjected to conventional nested and semi-nested PCR to amplify 16S rRNA and flaB gene fragments. Nine positive samples for both genes were sequenced, and seven and six sequences were of good quality for the 16S rRNA and flaB genes, respectively. The DNA of Borrelia spp. was detected in the insectivorous and fruit bats Artibeus lituratus, Carollia perspicillata, Glossophaga soricina, Phyllostomus discolor, and Uroderma sp. The 16S rRNA gene sequences showed 97.66–98.47% identity with “Borrelia sp. clone Omi3,” “Borrelia sp. RT1S,” and Borrelia sp. 2374; the closest identities for the flaB gene were 94.02–98.04% with “Borrelia sp. Macaregua.” For the 16S rRNA gene, the phylogenetic analysis showed a grouping with “Candidatus Borrelia ivorensis” and “Ca. Borrelia africana,” and for the flaB gene showed a grouping with Borrelia sp. Macaregua and Borrelia sp. Potiretama. The pathogenic role of the Borrelia detected in this study is unknown. Conclusions: We describe the first molecular evidence of Borrelia spp. in the department of Córdoba, Colombia, highlighting that several bat species harbor Borrelia spirochetes. Graphical Abstract: [Figure not available: see fulltext.].

AB - Background: The genus Borrelia is composed of two well-defined monophyletic groups, the Borrelia burgdorferi sensu lato complex (Bb) and the relapsing fever (RF) group borreliae. Recently, a third group, associated with reptiles and echidnas, has been described. In general, RF group borreliae use rodents as reservoir hosts; although neotropical bats may also be involved as important hosts, with scarce knowledge regarding this association. The objective of this study was to detect the presence of Borrelia spp. DNA in bats from the department of Córdoba in northwest Colombia. Methods: During September 2020 and June 2021, 205 bats were captured in six municipalities of Córdoba department, Colombia. Specimens were identified using taxonomic keys and DNA was extracted from spleen samples. A Borrelia-specific real-time PCR was performed for the 16S rRNA gene. Fragments of the 16S rRNA and flaB genes were amplified in the positive samples by conventional PCR. The detected amplicons were sequenced by the Sanger method. Phylogenetic reconstruction was performed in IQ-TREE with maximum likelihood based on the substitution model TPM3+F+I+G4 with bootstrap values deduced from 1000 replicates. Results: Overall, 10.2% (21/205) of the samples were found positive by qPCR; of these, 81% (17/21) and 66.6% (14/21) amplified 16S rRNA and flaB genes, respectively. qPCR-positive samples were then subjected to conventional nested and semi-nested PCR to amplify 16S rRNA and flaB gene fragments. Nine positive samples for both genes were sequenced, and seven and six sequences were of good quality for the 16S rRNA and flaB genes, respectively. The DNA of Borrelia spp. was detected in the insectivorous and fruit bats Artibeus lituratus, Carollia perspicillata, Glossophaga soricina, Phyllostomus discolor, and Uroderma sp. The 16S rRNA gene sequences showed 97.66–98.47% identity with “Borrelia sp. clone Omi3,” “Borrelia sp. RT1S,” and Borrelia sp. 2374; the closest identities for the flaB gene were 94.02–98.04% with “Borrelia sp. Macaregua.” For the 16S rRNA gene, the phylogenetic analysis showed a grouping with “Candidatus Borrelia ivorensis” and “Ca. Borrelia africana,” and for the flaB gene showed a grouping with Borrelia sp. Macaregua and Borrelia sp. Potiretama. The pathogenic role of the Borrelia detected in this study is unknown. Conclusions: We describe the first molecular evidence of Borrelia spp. in the department of Córdoba, Colombia, highlighting that several bat species harbor Borrelia spirochetes. Graphical Abstract: [Figure not available: see fulltext.].

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Molecular evidence of Borrelia spp. in bats from Córdoba Department, northwest Colombia

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