Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris

Valerie Amann; Ann-Kathrin Kissmann; Vanessa Mildenberger; Imke Krebs; Julio A Perez-Erviti; Ernesto M Martell-Huguet; Anselmo J Otero-Gonzalez; Fidel Morales-Vicente; Gina P Rodríguez-Castaño; Carolina Firacative; Armando Rodríguez; Ludger Ständker; Tanja Weil; Barbara Spellerberg; Steffen Stenger; Frank Rosenau

doi:10.3390/ijms24129788

Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris

Valerie Amann, Ann-Kathrin Kissmann, Vanessa Mildenberger, Imke Krebs, Julio A Perez-Erviti, Ernesto M Martell-Huguet, Anselmo J Otero-Gonzalez, Fidel Morales-Vicente, Gina P Rodríguez-Castaño, Carolina Firacative, Armando Rodríguez, Ludger Ständker, Tanja Weil, Barbara Spellerberg, Steffen Stenger, Frank Rosenau

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2 Citas (Scopus)

Resumen

Los péptidos antimicrobianos (AMP) representan una prometedora clase de biomoléculas terapéuticas que muestran actividad antimicrobiana contra una amplia gama de microorganismos, incluidos patógenos potencialmente mortales. A diferencia de los AMP clásicos con actividad disruptiva de membranas, los nuevos péptidos con efecto específico antibiofilm están cobrando importancia, ya que los biofilms podrían ser la forma de vida más importante, especialmente para los patógenos, pues la interacción con los tejidos del huésped es crucial para el pleno desarrollo de su virulencia en caso de infección. Por ello, en un estudio anterior, dos derivados diméricos sintéticos (dímero paralelo 1 y dímero antiparalelo 2) del AMP Cm-p5 mostraron una inhibición específica de la formación de biopelículas de Candida auris. Aquí demostramos que estos derivados también son eficaces en función de la dosis contra las biopelículas de novo formadas por las levaduras patógenas C. albicans y C. parapsilosis. Además, la actividad de los péptidos se demostró incluso contra dos cepas de C. auris resistentes al fluconazol.

Idioma original	Inglés estadounidense
Número de artículo	9788
Páginas (desde-hasta)	1-14
Número de páginas	14
Publicación	International Journal of Molecular Sciences
Volumen	24
N.º	12
DOI	https://doi.org/10.3390/ijms24129788
Estado	Publicada - jun. 6 2023

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Microbiología (médica)

ODS de las Naciones Unidas

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Amann, V., Kissmann, A.-K., Mildenberger, V., Krebs, I., Perez-Erviti, J. A., Martell-Huguet, E. M., Otero-Gonzalez, A. J., Morales-Vicente, F., Rodríguez-Castaño, G. P., Firacative, C., Rodríguez, A., Ständker, L., Weil, T., Spellerberg, B., Stenger, S., & Rosenau, F. (2023). Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris. International Journal of Molecular Sciences, 24(12), 1-14. Artículo 9788. https://doi.org/10.3390/ijms24129788

@article{b5c5f89864b64580bec18e89445fe39c,

title = "Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris",

abstract = "Antimicrobial peptides (AMPs) represent a promising class of therapeutic biomolecules that show antimicrobial activity against a broad range of microorganisms, including life-threatening pathogens. In contrast to classic AMPs with membrane-disrupting activities, new peptides with a specific anti-biofilm effect are gaining in importance since biofilms could be the most important way of life, especially for pathogens, as the interaction with host tissues is crucial for the full development of their virulence in the event of infection. Therefore, in a previous study, two synthetic dimeric derivatives (parallel Dimer 1 and antiparallel Dimer 2) of the AMP Cm-p5 showed specific inhibition of the formation of Candida auris biofilms. Here we show that these derivatives are also dose-dependently effective against de novo biofilms that are formed by the widespread pathogenic yeasts C. albicans and C. parapsilosis. Moreover, the activity of the peptides was demonstrated even against two fluconazole-resistant strains of C. auris.",

author = "Valerie Amann and Ann-Kathrin Kissmann and Vanessa Mildenberger and Imke Krebs and Perez-Erviti, {Julio A} and Martell-Huguet, {Ernesto M} and Otero-Gonzalez, {Anselmo J} and Fidel Morales-Vicente and Rodr{\'i}guez-Casta{\~n}o, {Gina P} and Carolina Firacative and Armando Rodr{\'i}guez and Ludger St{\"a}ndker and Tanja Weil and Barbara Spellerberg and Steffen Stenger and Frank Rosenau",

year = "2023",

month = jun,

day = "6",

doi = "10.3390/ijms24129788",

language = "English (US)",

volume = "24",

pages = "1--14",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "12",

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Amann, V, Kissmann, A-K, Mildenberger, V, Krebs, I, Perez-Erviti, JA, Martell-Huguet, EM, Otero-Gonzalez, AJ, Morales-Vicente, F, Rodríguez-Castaño, GP, Firacative, C, Rodríguez, A, Ständker, L, Weil, T, Spellerberg, B, Stenger, S & Rosenau, F 2023, 'Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris', International Journal of Molecular Sciences, vol. 24, n.º 12, 9788, pp. 1-14. https://doi.org/10.3390/ijms24129788

Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris. / Amann, Valerie; Kissmann, Ann-Kathrin; Mildenberger, Vanessa et al.
En: International Journal of Molecular Sciences, Vol. 24, N.º 12, 9788, 06.06.2023, p. 1-14.

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris

AU - Amann, Valerie

AU - Kissmann, Ann-Kathrin

AU - Mildenberger, Vanessa

AU - Krebs, Imke

AU - Perez-Erviti, Julio A

AU - Martell-Huguet, Ernesto M

AU - Otero-Gonzalez, Anselmo J

AU - Morales-Vicente, Fidel

AU - Rodríguez-Castaño, Gina P

AU - Firacative, Carolina

AU - Rodríguez, Armando

AU - Ständker, Ludger

AU - Weil, Tanja

AU - Spellerberg, Barbara

AU - Stenger, Steffen

AU - Rosenau, Frank

PY - 2023/6/6

Y1 - 2023/6/6

N2 - Antimicrobial peptides (AMPs) represent a promising class of therapeutic biomolecules that show antimicrobial activity against a broad range of microorganisms, including life-threatening pathogens. In contrast to classic AMPs with membrane-disrupting activities, new peptides with a specific anti-biofilm effect are gaining in importance since biofilms could be the most important way of life, especially for pathogens, as the interaction with host tissues is crucial for the full development of their virulence in the event of infection. Therefore, in a previous study, two synthetic dimeric derivatives (parallel Dimer 1 and antiparallel Dimer 2) of the AMP Cm-p5 showed specific inhibition of the formation of Candida auris biofilms. Here we show that these derivatives are also dose-dependently effective against de novo biofilms that are formed by the widespread pathogenic yeasts C. albicans and C. parapsilosis. Moreover, the activity of the peptides was demonstrated even against two fluconazole-resistant strains of C. auris.

AB - Antimicrobial peptides (AMPs) represent a promising class of therapeutic biomolecules that show antimicrobial activity against a broad range of microorganisms, including life-threatening pathogens. In contrast to classic AMPs with membrane-disrupting activities, new peptides with a specific anti-biofilm effect are gaining in importance since biofilms could be the most important way of life, especially for pathogens, as the interaction with host tissues is crucial for the full development of their virulence in the event of infection. Therefore, in a previous study, two synthetic dimeric derivatives (parallel Dimer 1 and antiparallel Dimer 2) of the AMP Cm-p5 showed specific inhibition of the formation of Candida auris biofilms. Here we show that these derivatives are also dose-dependently effective against de novo biofilms that are formed by the widespread pathogenic yeasts C. albicans and C. parapsilosis. Moreover, the activity of the peptides was demonstrated even against two fluconazole-resistant strains of C. auris.

U2 - 10.3390/ijms24129788

DO - 10.3390/ijms24129788

M3 - Research Article

C2 - 37372935

SN - 1661-6596

VL - 24

SP - 1

EP - 14

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

IS - 12

M1 - 9788

ER -

Cm-p5 Peptide Dimers Inhibit Biofilms of Candida albicans Clinical Isolates, C. parapsilosis and Fluconazole-Resistant Mutants of C. auris

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