TY - JOUR
T1 - Characterisation of the Plasmodium vivax Pv38 antigen
AU - Mongui, Alvaro
AU - Angel, Diana I.
AU - Guzman, Catalina
AU - Vanegas, Magnolia
AU - Patarroyo, Manuel A.
N1 - Funding Information:
We thank Oswaldo Escobar, Gina Gallego and Javier Ardila for their technical support. We would also like to thank Nora Martinez for reviewing this manuscript. Special gratitude goes to Professor Manuel Elkin Patarroyo for his invaluable comments and suggestions. This research was supported by President of Colombia’s office and the “Instituto Colombiano para el Desarrollo de la Ciencia ‘Francisco Jose de Caldas’ (COLCIENCIAS)” contract RC-2008.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/11/14
Y1 - 2008/11/14
N2 - This study describes the identification and characterisation of Pv38, based on the available genomic sequence of Plasmodium vivax and previous studies done with its Plasmodium falciparum homologue: Pf38. Pv38 is a 355 amino acid long peptide encoded by a single exon gene, for which orthologous genes have been identified in other Plasmodium species by bioinformatic approaches. As for Pf38, Pv38 was found to contain a s48/45 domain which is usually found in proteins displayed on gametocytes surface. The association of Pv38 with detergent-resistant membranes (DRMs), its expression in mature blood stages of the parasite (mainly schizonts) and the detection of its recombinant protein by sera from Aotus monkeys previously exposed to the parasite, were here assessed to further characterise this new antigen.
AB - This study describes the identification and characterisation of Pv38, based on the available genomic sequence of Plasmodium vivax and previous studies done with its Plasmodium falciparum homologue: Pf38. Pv38 is a 355 amino acid long peptide encoded by a single exon gene, for which orthologous genes have been identified in other Plasmodium species by bioinformatic approaches. As for Pf38, Pv38 was found to contain a s48/45 domain which is usually found in proteins displayed on gametocytes surface. The association of Pv38 with detergent-resistant membranes (DRMs), its expression in mature blood stages of the parasite (mainly schizonts) and the detection of its recombinant protein by sera from Aotus monkeys previously exposed to the parasite, were here assessed to further characterise this new antigen.
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U2 - 10.1016/j.bbrc.2008.08.163
DO - 10.1016/j.bbrc.2008.08.163
M3 - Article
C2 - 18789893
AN - SCOPUS:52949134094
SN - 0006-291X
VL - 376
SP - 326
EP - 330
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -
