Expresión de TRF2 y GAPDH en el envejecimiento de las células del epitelio superficial del ovario in vitro

Translated title of the contribution: Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells

Research output: Contribution to journalArticlepeer-review

Abstract

GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.

Translated title of the contributionExpression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells
Original languageSpanish
Pages (from-to)219-228
Number of pages10
JournalRevista ciencias de la salud
Volume9
Issue number3
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Health(social science)

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