Well-defined regions of the Plasmodium falciparum reticulocyte binding protein homologue 4 mediate interaction with red blood cell membrane

Jeison García, Hernando Curtidor, Carlos G. Pinzón, Manuel A. Patarroyo, Magnolia Vanegas, Martha Forero, Manuel E. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

6 Citas (Scopus)

Resumen

Two widely studied parasite protein families are considered attractive targets for developing a fully effective antimalarial vaccine: the erythrocyte binding antigen (EBA) family defining a sialic acid-dependent invasion pathway, and reticulocyte-binding homologue (RH) proteins associated with sialic acid-independent red blood cell (RBC) invasion. In this study, the micronemal invasive PfRH4 protein was finely mapped using 20-mer-long synthetic peptides spanning the entire protein length to identify protein regions that establish high affinity interactions with human RBCs. Twenty conserved, mainly α-helical high-activity binding peptides (HABPs) with nanomolar dissociation constants and recognizing 32, 25, 22, and 20 kDaRBCmembrane molecules in a chymotrypsin and/or trypsin-sensitive manner were identified in this protein. Anti-PfRH4 rabbit sera and PfRH4 HABPs inhibited merozoite invasion in vitro, therefore suggesting the implication of these HABPs in Plasmodium falciparum invasion and supporting their inclusion in further structural and immunological studies to design potential components of a minimal subunit-based, multiantigenic, chemically synthesized antimalarial vaccine. ©2009 American Chemical Society.
Idioma originalEnglish (US)
Páginas (desde-hasta)811-821
Número de páginas11
PublicaciónJournal of Medicinal Chemistry
DOI
EstadoPublished - mar 1 2010

Huella dactilar

Reticulocytes
Plasmodium falciparum
Carrier Proteins
Erythrocytes
Cell Membrane
Peptides
Antimalarials
N-Acetylneuraminic Acid
Proteins
Vaccines
Merozoites
Parasites
Rabbits
Antigens
Serum

Citar esto

García, Jeison ; Curtidor, Hernando ; Pinzón, Carlos G. ; Patarroyo, Manuel A. ; Vanegas, Magnolia ; Forero, Martha ; Patarroyo, Manuel E. / Well-defined regions of the Plasmodium falciparum reticulocyte binding protein homologue 4 mediate interaction with red blood cell membrane. En: Journal of Medicinal Chemistry. 2010 ; pp. 811-821.
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abstract = "Two widely studied parasite protein families are considered attractive targets for developing a fully effective antimalarial vaccine: the erythrocyte binding antigen (EBA) family defining a sialic acid-dependent invasion pathway, and reticulocyte-binding homologue (RH) proteins associated with sialic acid-independent red blood cell (RBC) invasion. In this study, the micronemal invasive PfRH4 protein was finely mapped using 20-mer-long synthetic peptides spanning the entire protein length to identify protein regions that establish high affinity interactions with human RBCs. Twenty conserved, mainly α-helical high-activity binding peptides (HABPs) with nanomolar dissociation constants and recognizing 32, 25, 22, and 20 kDaRBCmembrane molecules in a chymotrypsin and/or trypsin-sensitive manner were identified in this protein. Anti-PfRH4 rabbit sera and PfRH4 HABPs inhibited merozoite invasion in vitro, therefore suggesting the implication of these HABPs in Plasmodium falciparum invasion and supporting their inclusion in further structural and immunological studies to design potential components of a minimal subunit-based, multiantigenic, chemically synthesized antimalarial vaccine. {\circledC}2009 American Chemical Society.",
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Well-defined regions of the Plasmodium falciparum reticulocyte binding protein homologue 4 mediate interaction with red blood cell membrane. / García, Jeison; Curtidor, Hernando; Pinzón, Carlos G.; Patarroyo, Manuel A.; Vanegas, Magnolia; Forero, Martha; Patarroyo, Manuel E.

En: Journal of Medicinal Chemistry, 01.03.2010, p. 811-821.

Resultado de la investigación: Contribución a RevistaArtículo

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T1 - Well-defined regions of the Plasmodium falciparum reticulocyte binding protein homologue 4 mediate interaction with red blood cell membrane

AU - García, Jeison

AU - Curtidor, Hernando

AU - Pinzón, Carlos G.

AU - Patarroyo, Manuel A.

AU - Vanegas, Magnolia

AU - Forero, Martha

AU - Patarroyo, Manuel E.

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