Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units

Carolina Hernández, Camilo Salazar, Helena Brochero, Aníbal Teherán, Luz Stella Buitrago, Mauricio Vera, Hugo Soto, Zulibeth Florez-Rivadeneira, Sussane Ardila, Gabriel Parra-Henao, Juan David Ramírez

Resultado de la investigación: Contribución a RevistaArtículo

6 Citas (Scopus)

Resumen

© 2016 The Author(s).Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2% and presented statistical associations with the departments Meta (OR: 2.65; 95% CI: 1.69-4.17) and Guajira (OR: 2.13; 95% CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95% CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95% CI: 1.51-3.82) and T. maculata (OR: 2.09; 95% CI: 1.02-4.29); females (OR: 2.05; 95% CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95% CI: 1.85-11.69) and human blood (OR: 1.55; 95% CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3%), TcII (6.7%), TcIII (8.7%), TcIV (4.0%) and TcV (6.0%). Across the samples typed as TcI, we detected TcIDom (19%) and sylvatic TcI (75%). The frequencies of feeding sources were 59.4% (human blood); 11.2% (hen); 9.6% (bat); 5.6% (opossum); 5.1% (mouse); 4.1% (dog); 3.0% (rodent); 1.0% (armadillo); and 1.0% (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia.
Idioma originalEnglish (US)
PublicaciónParasites and Vectors
DOI
EstadoPublished - ene 12 2016

Huella dactilar

Parasitic Diseases
Colombia
Trypanosoma cruzi
Insects
Opossums
Infection
Panstrongylus
Logistic Models
Rhodnius
Triatoma
Armadillos
Chagas Disease
Rodentia
Genotype
Demography
Dogs
Food

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Hernández, Carolina ; Salazar, Camilo ; Brochero, Helena ; Teherán, Aníbal ; Buitrago, Luz Stella ; Vera, Mauricio ; Soto, Hugo ; Florez-Rivadeneira, Zulibeth ; Ardila, Sussane ; Parra-Henao, Gabriel ; Ramírez, Juan David. / Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units. En: Parasites and Vectors. 2016.
@article{96a0f5e7613644d0a658d26b567cd041,
title = "Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units",
abstract = "{\circledC} 2016 The Author(s).Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2{\%} and presented statistical associations with the departments Meta (OR: 2.65; 95{\%} CI: 1.69-4.17) and Guajira (OR: 2.13; 95{\%} CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95{\%} CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95{\%} CI: 1.51-3.82) and T. maculata (OR: 2.09; 95{\%} CI: 1.02-4.29); females (OR: 2.05; 95{\%} CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95{\%} CI: 1.85-11.69) and human blood (OR: 1.55; 95{\%} CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3{\%}), TcII (6.7{\%}), TcIII (8.7{\%}), TcIV (4.0{\%}) and TcV (6.0{\%}). Across the samples typed as TcI, we detected TcIDom (19{\%}) and sylvatic TcI (75{\%}). The frequencies of feeding sources were 59.4{\%} (human blood); 11.2{\%} (hen); 9.6{\%} (bat); 5.6{\%} (opossum); 5.1{\%} (mouse); 4.1{\%} (dog); 3.0{\%} (rodent); 1.0{\%} (armadillo); and 1.0{\%} (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia.",
author = "Carolina Hern{\'a}ndez and Camilo Salazar and Helena Brochero and An{\'i}bal Teher{\'a}n and Buitrago, {Luz Stella} and Mauricio Vera and Hugo Soto and Zulibeth Florez-Rivadeneira and Sussane Ardila and Gabriel Parra-Henao and Ram{\'i}rez, {Juan David}",
year = "2016",
month = "1",
day = "12",
doi = "10.1186/s13071-016-1907-5",
language = "English (US)",
journal = "Parasites and Vectors",
issn = "1756-3305",
publisher = "BioMed Central",

}

Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units. / Hernández, Carolina; Salazar, Camilo; Brochero, Helena; Teherán, Aníbal; Buitrago, Luz Stella; Vera, Mauricio; Soto, Hugo; Florez-Rivadeneira, Zulibeth; Ardila, Sussane; Parra-Henao, Gabriel; Ramírez, Juan David.

En: Parasites and Vectors, 12.01.2016.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Untangling the transmission dynamics of primary and secondary vectors of Trypanosoma cruzi in Colombia: Parasite infection, feeding sources and discrete typing units

AU - Hernández, Carolina

AU - Salazar, Camilo

AU - Brochero, Helena

AU - Teherán, Aníbal

AU - Buitrago, Luz Stella

AU - Vera, Mauricio

AU - Soto, Hugo

AU - Florez-Rivadeneira, Zulibeth

AU - Ardila, Sussane

AU - Parra-Henao, Gabriel

AU - Ramírez, Juan David

PY - 2016/1/12

Y1 - 2016/1/12

N2 - © 2016 The Author(s).Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2% and presented statistical associations with the departments Meta (OR: 2.65; 95% CI: 1.69-4.17) and Guajira (OR: 2.13; 95% CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95% CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95% CI: 1.51-3.82) and T. maculata (OR: 2.09; 95% CI: 1.02-4.29); females (OR: 2.05; 95% CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95% CI: 1.85-11.69) and human blood (OR: 1.55; 95% CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3%), TcII (6.7%), TcIII (8.7%), TcIV (4.0%) and TcV (6.0%). Across the samples typed as TcI, we detected TcIDom (19%) and sylvatic TcI (75%). The frequencies of feeding sources were 59.4% (human blood); 11.2% (hen); 9.6% (bat); 5.6% (opossum); 5.1% (mouse); 4.1% (dog); 3.0% (rodent); 1.0% (armadillo); and 1.0% (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia.

AB - © 2016 The Author(s).Background: Trypanosoma cruzi is the causative agent of Chagas disease. Due to its genetic diversity has been classified into six Discrete Typing Units (DTUs) in association with transmission cycles. In Colombia, natural T. cruzi infection has been detected in 15 triatomine species. There is scarce information regarding the infection rates, DTUs and feeding preferences of secondary vectors. Therefore, the aim of this study was to determine T. cruzi infection rates, parasite DTU, ecotopes, insect stages, geographical location and bug feeding preferences across six different triatomine species. Methods: A total of 245 insects were collected in seven departments of Colombia. We conducted molecular detection and genotyping of T. cruzi with subsequent identification of food sources. The frequency of infection, DTUs, TcI genotypes and feeding sources were plotted across the six species studied. A logistic regression model risk was estimated with insects positive for T. cruzi according to demographic and eco-epidemiological characteristics. Results: We collected 85 specimens of Panstrongylus geniculatus, 77 Rhodnius prolixus, 37 R. pallescens, 34 Triatoma maculata, 8 R. pictipes and 4 T. dimidiata. The overall T. cruzi infection rate was 61.2% and presented statistical associations with the departments Meta (OR: 2.65; 95% CI: 1.69-4.17) and Guajira (OR: 2.13; 95% CI: 1.16-3.94); peridomestic ecotope (OR: 2.52: 95% CI: 1.62-3.93); the vector species P. geniculatus (OR: 2.40; 95% CI: 1.51-3.82) and T. maculata (OR: 2.09; 95% CI: 1.02-4.29); females (OR: 2.05; 95% CI: 1.39-3.04) and feeding on opossum (OR: 3.15; 95% CI: 1.85-11.69) and human blood (OR: 1.55; 95% CI: 1.07-2.24). Regarding the DTUs, we observed TcI (67.3%), TcII (6.7%), TcIII (8.7%), TcIV (4.0%) and TcV (6.0%). Across the samples typed as TcI, we detected TcIDom (19%) and sylvatic TcI (75%). The frequencies of feeding sources were 59.4% (human blood); 11.2% (hen); 9.6% (bat); 5.6% (opossum); 5.1% (mouse); 4.1% (dog); 3.0% (rodent); 1.0% (armadillo); and 1.0% (cow). Conclusions: New scenarios of T. cruzi transmission caused by secondary and sylvatic vectors are considered. The findings of sylvatic DTUs from bugs collected in domestic and peridomestic ecotopes confirms the emerging transmission scenarios in Colombia.

U2 - 10.1186/s13071-016-1907-5

DO - 10.1186/s13071-016-1907-5

M3 - Article

JO - Parasites and Vectors

JF - Parasites and Vectors

SN - 1756-3305

ER -