Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica: Identification of genes and pathways involved in conferring immunoprotection in a murine model

Jose Rojas-Caraballo, Julio López-Abán, Darwin Andrés Moreno-Pérez, Belén Vicente, Pedro Fernández-Soto, Esther del Olmo, Manuel Alfonso Patarroyo, Antonio Muro

Resultado de la investigación: Contribución a RevistaArtículo

2 Citas (Scopus)

Resumen

© 2017 The Author(s).Background: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F. hepatica. Methods: The mice were immunised with synthetic peptides containing B- and T-cell epitopes, which are derived from F. hepatica cathepsin B and amoebapore proteins, as novel vaccine candidates against F. hepatica formulated in an adjuvant adaptation vaccination system; they were experimentally challenged with F. hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. Data was then background corrected, normalised and the expression signal was calculated. The Ingenuity Pathway Analysis tool was then used for analysing differentially expressed gene identifiers for annotating bio-functions and constructing and visualising molecular interaction networks. Results: Mice immunised with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes, 168 genes being up-regulated and 652 genes being down-regulated, p value
Idioma originalEnglish (US)
PublicaciónBMC Infectious Diseases
DOI
EstadoPublished - ene 23 2017

Huella dactilar

Fasciola hepatica
Gene Expression Profiling
Immunization
Gene Expression
Peptide T
T-Lymphocyte Epitopes
Vaccines
Genes
Metacercariae
B-Lymphocyte Epitopes
Cathepsin B
Herbivory
Foodborne Diseases
Transcriptome
Epitopes
Vaccination
Spleen
Survival Rate
RNA
Technology

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Rojas-Caraballo, Jose ; López-Abán, Julio ; Moreno-Pérez, Darwin Andrés ; Vicente, Belén ; Fernández-Soto, Pedro ; del Olmo, Esther ; Patarroyo, Manuel Alfonso ; Muro, Antonio. / Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica: Identification of genes and pathways involved in conferring immunoprotection in a murine model. En: BMC Infectious Diseases. 2017.
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title = "Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica: Identification of genes and pathways involved in conferring immunoprotection in a murine model",
abstract = "{\circledC} 2017 The Author(s).Background: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F. hepatica. Methods: The mice were immunised with synthetic peptides containing B- and T-cell epitopes, which are derived from F. hepatica cathepsin B and amoebapore proteins, as novel vaccine candidates against F. hepatica formulated in an adjuvant adaptation vaccination system; they were experimentally challenged with F. hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. Data was then background corrected, normalised and the expression signal was calculated. The Ingenuity Pathway Analysis tool was then used for analysing differentially expressed gene identifiers for annotating bio-functions and constructing and visualising molecular interaction networks. Results: Mice immunised with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes, 168 genes being up-regulated and 652 genes being down-regulated, p value",
author = "Jose Rojas-Caraballo and Julio L{\'o}pez-Ab{\'a}n and Moreno-P{\'e}rez, {Darwin Andr{\'e}s} and Bel{\'e}n Vicente and Pedro Fern{\'a}ndez-Soto and {del Olmo}, Esther and Patarroyo, {Manuel Alfonso} and Antonio Muro",
year = "2017",
month = "1",
day = "23",
doi = "10.1186/s12879-017-2205-3",
language = "English (US)",
journal = "BMC Infectious Diseases",
issn = "1471-2334",
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Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica: Identification of genes and pathways involved in conferring immunoprotection in a murine model. / Rojas-Caraballo, Jose; López-Abán, Julio; Moreno-Pérez, Darwin Andrés; Vicente, Belén; Fernández-Soto, Pedro; del Olmo, Esther; Patarroyo, Manuel Alfonso; Muro, Antonio.

En: BMC Infectious Diseases, 23.01.2017.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Transcriptome profiling of gene expression during immunisation trial against Fasciola hepatica: Identification of genes and pathways involved in conferring immunoprotection in a murine model

AU - Rojas-Caraballo, Jose

AU - López-Abán, Julio

AU - Moreno-Pérez, Darwin Andrés

AU - Vicente, Belén

AU - Fernández-Soto, Pedro

AU - del Olmo, Esther

AU - Patarroyo, Manuel Alfonso

AU - Muro, Antonio

PY - 2017/1/23

Y1 - 2017/1/23

N2 - © 2017 The Author(s).Background: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F. hepatica. Methods: The mice were immunised with synthetic peptides containing B- and T-cell epitopes, which are derived from F. hepatica cathepsin B and amoebapore proteins, as novel vaccine candidates against F. hepatica formulated in an adjuvant adaptation vaccination system; they were experimentally challenged with F. hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. Data was then background corrected, normalised and the expression signal was calculated. The Ingenuity Pathway Analysis tool was then used for analysing differentially expressed gene identifiers for annotating bio-functions and constructing and visualising molecular interaction networks. Results: Mice immunised with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes, 168 genes being up-regulated and 652 genes being down-regulated, p value

AB - © 2017 The Author(s).Background: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F. hepatica. Methods: The mice were immunised with synthetic peptides containing B- and T-cell epitopes, which are derived from F. hepatica cathepsin B and amoebapore proteins, as novel vaccine candidates against F. hepatica formulated in an adjuvant adaptation vaccination system; they were experimentally challenged with F. hepatica metacercariae. Spleen RNA from mice immunised with the highest protection-inducing synthetic peptides was isolated, amplified and labelled using Affymetrix standardised protocols. Data was then background corrected, normalised and the expression signal was calculated. The Ingenuity Pathway Analysis tool was then used for analysing differentially expressed gene identifiers for annotating bio-functions and constructing and visualising molecular interaction networks. Results: Mice immunised with a combination of three peptides containing T-cell epitopes induced high protection against experimental challenge according to survival rates and hepatic damage scores. It also induced differential expression of 820 genes, 168 genes being up-regulated and 652 genes being down-regulated, p value

U2 - 10.1186/s12879-017-2205-3

DO - 10.1186/s12879-017-2205-3

M3 - Article

JO - BMC Infectious Diseases

JF - BMC Infectious Diseases

SN - 1471-2334

ER -