Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis

Título traducido de la contribución: Toxoplasma gondii: patrón de reconocimiento de péptidos P30 en toxoplasmosis humana

Nestor Cardona, Alejandra de-la-Torre, Heber Siachoque, Manuel A. Patarroyo, Jorge E. Gomez-Marin

Resultado de la investigación: Contribución a RevistaArtículo

18 Citas (Scopus)

Resumen

En este estudio, la reactividad del suero humano contra nueve péptidos derivados de la proteína Toxoplasma gondii P30 se evaluó mediante ELISA en pacientes con diferentes formas clínicas de toxoplasmosis. Igual que se ha informado en ratones, los sueros de pacientes con toxoplasmosis asintomática congénita, ocular y crónica reconocieron con mayor fuerza péptidos del extremo carboxilo de la proteína, siendo el péptido 2017 (aminoácidos 301-320) el más fuertemente reconocido por el suero de pacientes con ocular toxoplasmosis. Las muestras de suero recogidas de 13 pacientes sin infección ocular, 13 con cicatrices coriorretinianas inactivas, 6 con infección ocular activa y 10 personas seronegativas fueron examinadas para detectar IgG anti-2017. El péptido 2017 fue reconocido por todas las muestras de los pacientes pero no por los sueros de individuos con T. gondii-seronegativos. No se encontraron diferencias estadísticamente significativas entre los niveles de absorbancia de grupos con y sin lesiones o con lesiones oculares activas o inactivas, según lo determinado por ANOVA
Idioma originalEnglish (US)
Páginas (desde-hasta)199-202
Número de páginas4
PublicaciónExperimental Parasitology
Volumen123
N.º2
DOI
EstadoPublished - oct 1 2009

Huella dactilar

Toxoplasmosis
Toxoplasma
Peptides
Eye Infections
Serum
Ocular Toxoplasmosis
Cicatrix
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Amino Acids
Proteins

Citar esto

Cardona, Nestor ; de-la-Torre, Alejandra ; Siachoque, Heber ; Patarroyo, Manuel A. ; Gomez-Marin, Jorge E. / Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis. En: Experimental Parasitology. 2009 ; Vol. 123, N.º 2. pp. 199-202.
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abstract = "In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more strongly peptides from the protein's carboxy-terminus, being peptide 2017 (amino acids 301-320) the one most strongly recognized by sera from patients with ocular toxoplasmosis. Serum samples collected from 13 patients without ocular infection, 13 with inactive chorioretinal scars, 6 with active ocular infection and 10 seronegative individuals were then screened for anti-2017 IgG. Peptide 2017 was recognized by all patients' samples but not by sera from T. gondii-seronegative individuals. No statistically significant differences were found between the absorbance levels of groups with and without lesions or with active or inactive ocular lesions, as determined by ANOVA. {\circledC} 2009 Elsevier Inc. All rights reserved.",
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Toxoplasma gondii: P30 peptides recognition pattern in human toxoplasmosis. / Cardona, Nestor; de-la-Torre, Alejandra; Siachoque, Heber; Patarroyo, Manuel A.; Gomez-Marin, Jorge E.

En: Experimental Parasitology, Vol. 123, N.º 2, 01.10.2009, p. 199-202.

Resultado de la investigación: Contribución a RevistaArtículo

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AU - Cardona, Nestor

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AU - Gomez-Marin, Jorge E.

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N2 - In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more strongly peptides from the protein's carboxy-terminus, being peptide 2017 (amino acids 301-320) the one most strongly recognized by sera from patients with ocular toxoplasmosis. Serum samples collected from 13 patients without ocular infection, 13 with inactive chorioretinal scars, 6 with active ocular infection and 10 seronegative individuals were then screened for anti-2017 IgG. Peptide 2017 was recognized by all patients' samples but not by sera from T. gondii-seronegative individuals. No statistically significant differences were found between the absorbance levels of groups with and without lesions or with active or inactive ocular lesions, as determined by ANOVA. © 2009 Elsevier Inc. All rights reserved.

AB - In this study, human sera reactivity against nine peptides derived from the Toxoplasma gondii P30 protein was assessed by ELISA in patients with different clinical forms of toxoplasmosis. Same as has been reported in mice, sera from congenital, ocular and chronic asymptomatic toxoplasmosis patients recognized more strongly peptides from the protein's carboxy-terminus, being peptide 2017 (amino acids 301-320) the one most strongly recognized by sera from patients with ocular toxoplasmosis. Serum samples collected from 13 patients without ocular infection, 13 with inactive chorioretinal scars, 6 with active ocular infection and 10 seronegative individuals were then screened for anti-2017 IgG. Peptide 2017 was recognized by all patients' samples but not by sera from T. gondii-seronegative individuals. No statistically significant differences were found between the absorbance levels of groups with and without lesions or with active or inactive ocular lesions, as determined by ANOVA. © 2009 Elsevier Inc. All rights reserved.

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DO - 10.1016/j.exppara.2009.06.017

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JF - Experimental Parasitology

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