TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in northwestern Colombians

Luis M. Gomez, Edward A. Ruiz-Narváez, Ricardo Pineda-Tamayo, Adriana Rojas-Villarraga, Juan Manuel Anaya

Resultado de la investigación: Contribución a RevistaArtículo

4 Citas (Scopus)

Resumen

Objective: To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population. Methods: This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF microsatellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques. Results: By unconditional logistic regression analysis, the TNFa6 allele (OR= 2.37, 95%CI 1.07-5.24) and the TNFb4 allele (OR= 3.01, 95%CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR = 5.07, 95%CI 1.14-22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G. Conclusion: These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA. © Copyright Clinical and Experimental Rheumatology 2007.
Idioma originalEnglish (US)
Páginas (desde-hasta)443-448
Número de páginas6
PublicaciónClinical and Experimental Rheumatology
EstadoPublished - may 1 2007

Huella dactilar

Microsatellite Repeats
Rheumatoid Arthritis
Tumor Necrosis Factor-alpha
Alleles
Linkage Disequilibrium
HLA-DQ Antigens
HLA-DRB1 Chains
HLA-DR Antigens
Haplotypes
Joints
Logistic Models
Regression Analysis
Polymerase Chain Reaction
Population

Citar esto

Gomez, Luis M. ; Ruiz-Narváez, Edward A. ; Pineda-Tamayo, Ricardo ; Rojas-Villarraga, Adriana ; Anaya, Juan Manuel. / TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in northwestern Colombians. En: Clinical and Experimental Rheumatology. 2007 ; pp. 443-448.
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abstract = "Objective: To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population. Methods: This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF microsatellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques. Results: By unconditional logistic regression analysis, the TNFa6 allele (OR= 2.37, 95{\%}CI 1.07-5.24) and the TNFb4 allele (OR= 3.01, 95{\%}CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR = 5.07, 95{\%}CI 1.14-22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G. Conclusion: These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA. {\circledC} Copyright Clinical and Experimental Rheumatology 2007.",
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TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in northwestern Colombians. / Gomez, Luis M.; Ruiz-Narváez, Edward A.; Pineda-Tamayo, Ricardo; Rojas-Villarraga, Adriana; Anaya, Juan Manuel.

En: Clinical and Experimental Rheumatology, 01.05.2007, p. 443-448.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - TNF microsatellites polymorphism is associated with rheumatoid arthritis. Confirming evidence in northwestern Colombians

AU - Gomez, Luis M.

AU - Ruiz-Narváez, Edward A.

AU - Pineda-Tamayo, Ricardo

AU - Rojas-Villarraga, Adriana

AU - Anaya, Juan Manuel

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N2 - Objective: To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population. Methods: This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF microsatellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques. Results: By unconditional logistic regression analysis, the TNFa6 allele (OR= 2.37, 95%CI 1.07-5.24) and the TNFb4 allele (OR= 3.01, 95%CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR = 5.07, 95%CI 1.14-22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G. Conclusion: These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA. © Copyright Clinical and Experimental Rheumatology 2007.

AB - Objective: To examine the contribution of tumor necrosis factor alpha (TNF) microsatellite (a to e) polymorphism to the genetic risk of developing rheumatoid arthritis (RA) in a northwestern Colombian population. Methods: This was an association study in which 108 RA patients and 222 matched individuals were enrolled. HLA-DRB1 and DQB1 polymorphisms were evaluated to examine for linkage disequilibrium between these loci and TNF microsatellites. Genotyping was performed using denaturing polyacrylamide gels and polymerase chain reaction-sequence techniques. Results: By unconditional logistic regression analysis, the TNFa6 allele (OR= 2.37, 95%CI 1.07-5.24) and the TNFb4 allele (OR= 3.01, 95%CI 1.07-9.00) were observed to be associated with disease. These associations were independent of HLA-DR and HLA-DQ since linkage disequilibrium between HLA class II and TNF microsatellites was not observed. In addition, patients with the TNFa8 allele had a five times greater risk of developing extra-articular manifestations as compared to patients without this allele (OR = 5.07, 95%CI 1.14-22.52), regardless of age and the duration of disease. Haplotype analysis disclosed a protective effect for TNFa7/b7/c1/d4/e3/-308G/-238G. Conclusion: These results confirm that the TNF locus exerts a primary influence on both susceptibility to and the severity of RA. © Copyright Clinical and Experimental Rheumatology 2007.

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