Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region

Título traducido de la contribución: Tipificación de secuencias multilocales de los agentes de criptococosis en la cuenca del Río Negro, región amazónica brasileña.

Fabio Brito-Santos, Luciana Trilles, Carolina Firacative, Wieland Meyer, Marília Martins Nishikawa, Angela Cristina Veríssimo Junqueira , Amanda Coutinho de Souza, Filipe Anibal Carvalho-Costa, Bodo Wanke, Márcia dos Samntos Lazéra

Resultado de la investigación: Tipos de Contribuciónes en ConferenciaAfiche

Resumen

Objetivo: La criptococosis es una micosis sistémica potencialmente mortal que afecta a seres humanos y animales en todo el mundo. La enfermedad se adquiere por inhalación de propágulos infecciosos de los complejos de especies de Cryptococcus neoformans/C. gattii procedentes del medio ambiente. Los estudios ambientales pioneros sobre la criptococosis en el SIDA en África Central y en Brasil demostraron que las personas que viven en viviendas contaminadas corren el riesgo de contraer la criptococosis por la contaminación en interiores. En el corazón de la selva amazónica, un estudio reveló la presencia de C. gattii VGII en muestras de polvo de viviendas y postes de madera en un asentamiento humano remoto en la microrregión de Río Negro, Amazonas, Brasil. La alta densidad de las colonias criptocócicas sugirió que los habitantes locales están expuestos diariamente a la levadura. En este estudio, revelamos nuevas evidencias de C. neoformans y C. gattii 7 de 79 viviendas humanas investigadas y dos de 10 postes de madera en la cuenca del Río Negro, Métodos Amazónicos Brasileños: Se subtiparon 48 aislados de C. gattii y 18 aislados de C. neoformans utilizando los resultados del esquema de consenso MLST de ISHAM: En una de las viviendas positivas (An/P9) se encontraron 200 UFC/g de colonias de color marrón oscuro, identificadas como C. gattii VGII, y en otra vivienda (Re/P15) se encontraron 300 UFC/g de colonias de color marrón oscuro, identificadas como C. gattii VGII y C. neoformans VNI. Los subtipos más comunes de C. gattii fueron ST7 (Vancouver Island Outbreak subtipo VGIIb, 24/48) y ST20 (Vancouver Island Outbreak subtipo VGIIa, 11/48). Entre los 18 C. neoformans (VNI) se identificaron aislados ST93 y ST5, mostrando una población clonal. El análisis del tipo de apareamiento demostró que todos los aislados eran MATα Conclusión: Los subtipos MLST identificados de los aislados ambientales en la microrregión de Río Negro indican que el polvo domiciliario y los postes de madera son una fuente potencial de infección humana con diferentes subtipos de C. gattii VGII y C. neoformans VNI MATα y confirmaron el vínculo entre los asentamientos humanos remotos en la cuenca del río Negro y el brote de criptococosis del Pacífico Noroccidental.
Idioma originalEnglish (US)
PáginasS84
Número de páginas1
EstadoPublished - jun 6 2018
Evento20th Congress of the International Society for Human and Animal Mycology - Amsterdam
Duración: jun 30 2018jul 4 2018
https://www.isham2018.org/en/Home_10_6_12.html

Conference

Conference20th Congress of the International Society for Human and Animal Mycology
Título abreviado20th Congress ISHAM
PaísNetherlands
CiudadAmsterdam
Período6/30/187/4/18
Dirección de internet

Citar esto

Brito-Santos, F., Trilles, L., Firacative, C., Meyer, W., Nishikawa, M. M., Veríssimo Junqueira , A. C., ... dos Samntos Lazéra, M. (2018). Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. S84. Sesión de cárteles presentada en 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, .
Brito-Santos, Fabio ; Trilles, Luciana ; Firacative, Carolina ; Meyer, Wieland ; Nishikawa, Marília Martins ; Veríssimo Junqueira , Angela Cristina ; Coutinho de Souza, Amanda ; Carvalho-Costa, Filipe Anibal ; Wanke, Bodo ; dos Samntos Lazéra, Márcia . / Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. Sesión de cárteles presentada en 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, .1 p.
@conference{23e251ebbae1433f842de566ec14ca95,
title = "Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region",
abstract = "Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.",
author = "Fabio Brito-Santos and Luciana Trilles and Carolina Firacative and Wieland Meyer and Nishikawa, {Mar{\'i}lia Martins} and {Ver{\'i}ssimo Junqueira}, {Angela Cristina} and {Coutinho de Souza}, Amanda and Carvalho-Costa, {Filipe Anibal} and Bodo Wanke and {dos Samntos Laz{\'e}ra}, M{\'a}rcia",
year = "2018",
month = "6",
day = "6",
language = "English (US)",
pages = "S84",
note = "20th Congress of the International Society for Human and Animal Mycology, 20th Congress ISHAM ; Conference date: 30-06-2018 Through 04-07-2018",
url = "https://www.isham2018.org/en/Home_10_6_12.html",

}

Brito-Santos, F, Trilles, L, Firacative, C, Meyer, W, Nishikawa, MM, Veríssimo Junqueira , AC, Coutinho de Souza, A, Carvalho-Costa, FA, Wanke, B & dos Samntos Lazéra, M 2018, 'Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region', Amsterdam, 6/30/18 - 7/4/18, pp. S84.

Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. / Brito-Santos, Fabio; Trilles, Luciana; Firacative, Carolina; Meyer, Wieland; Nishikawa, Marília Martins; Veríssimo Junqueira , Angela Cristina; Coutinho de Souza, Amanda ; Carvalho-Costa, Filipe Anibal; Wanke, Bodo ; dos Samntos Lazéra, Márcia .

2018. S84 Sesión de cárteles presentada en 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, .

Resultado de la investigación: Tipos de Contribuciónes en ConferenciaAfiche

TY - CONF

T1 - Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region

AU - Brito-Santos, Fabio

AU - Trilles, Luciana

AU - Firacative, Carolina

AU - Meyer, Wieland

AU - Nishikawa, Marília Martins

AU - Veríssimo Junqueira , Angela Cristina

AU - Coutinho de Souza, Amanda

AU - Carvalho-Costa, Filipe Anibal

AU - Wanke, Bodo

AU - dos Samntos Lazéra, Márcia

PY - 2018/6/6

Y1 - 2018/6/6

N2 - Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.

AB - Objective: Cryptococcosis is a life-threatening systemic mycosis affecting humans and animals worldwide. The disease is acquired by inhalation of infectious propagules of the Cryptococcus neoformans/C. gattii species complexes from the environment. Pioneering environmental studies on cryptococcosis in AIDS in Central Africa and in Brazil demonstrated that individuals living in contaminated dwellings are at risk to acquire cryptococcosis from indoor contamination. In the heart of the Amazon rainforest, a study revealed the occurrence of C. gattii VGII in dust samples from dwellings and wood poles in a remote human settlement in the Rio Negro Microregion, Amazon, Brazil. The high density of cryptococcal colonies suggested that the local inhabitants are daily exposed to the yeast. In this study, we disclosed new evidences of C. neoformans and C. gattii 7 out of 79 investigated human dwellings and two out of 10 wood poles in the Rio Negro Basin, Brazilian Amazon Methods: Forty-eight C. gattii and 18 C. neoformans isolates were subtyped using the ISHAM MLST consensus scheme Results: In one of the positive dwelling (An/P9) 200 CFU/g of dark brown colonies were found, and identified as C. gattii VGII and in another dwelling (Re/P15) 300 CFU/g of dark brown colonies were found and identified as C. gattii VGII and C. neoformans VNI. The most common C. gattii subtypes were ST7 (Vancouver Island Outbreak subtype VGIIb, 24/48) and ST20 (Vancouver Island Outbreak subtype VGIIa, 11/48). Amongst the 18 C. neoformans (VNI) isolates ST93 and ST5 were identified, showing a clonal population. Mating type analysis demonstrated all isolates were MATα. Conclusion: The identified MLST subtypes from the environmental isolates in the microregion of Rio Negro indicate domiciliary dust as well as wood poles as a potential source for human infection with different subtypes of C. gattii VGII and C. neoformans VNI MATα and confirmed the link between remote human settlements in the Rio Negro river basin and the Pacific North West outbreak of cryptococcosis.

M3 - Poster

SP - S84

ER -

Brito-Santos F, Trilles L, Firacative C, Meyer W, Nishikawa MM, Veríssimo Junqueira AC y otros. Multilocus sequence typing of the agents of cryptococcosis in the Rio Negro river basin, Brazilian Amazon region. 2018. Sesión de cárteles presentada en 20th Congress of the International Society for Human and Animal Mycology, Amsterdam, .