The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence

Rafael G. Lopes; Julián E. Muñoz; Ludmila M. Barros; Sergio L. Alves-Jr; Carlos P. Taborda; Boris U. Stambuk

doi:10.1590/0074-02760200401

The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence

Título traducido de la contribución: La trehalasa ácida secretada codificada por el gen CgATH1 está involucrada en la virulencia de Candida glabrata

Rafael G. Lopes
, Julián E. Muñoz
, Ludmila M. Barros
, Sergio L. Alves-Jr
, Carlos P. Taborda
, Boris U. Stambuk

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

5 Citas (Scopus)

Resumen

ANTECEDENTES

La levadura Candida glabrata es la segunda causa de candidiasis en el mundo. Se diferencia de otras levaduras en que asimila únicamente glucosa y trehalosa (característica utilizada en pruebas de identificación rápida de este patógeno) secretando al medio una trehalasa ácida altamente activa codificada por el gen CgATH1.

OBJETIVO

Este estudio tuvo como objetivo caracterizar la función de la trehalasa ácida en la fisiopatología de C. glabrata.

MÉTODOS

Se realizó una deleción génica para obtener una cepa mutante ath1Δ y se verificó la capacidad de la cepa ath1Δ para crecer en trehalasa, o la presencia de actividad de trehalasa en las células de levadura ath1Δ. También probamos la virulencia de la cepa ath1Δ en un modelo murino de infección.

RESULTADOS

La cepa mutante ath1Δ crece normalmente en presencia de glucosa, pero pierde su capacidad de crecer en trehalosa. Debido a la alta actividad de trehalasa ácida presente en las células de tipo salvaje, la actividad de trehalasa neutra citoplasmática solo se detecta en la cepa ath1Δ. También observamos una virulencia significativamente menor de la cepa ath1Δ en un modelo murino de infección con ratones normales o inmunodeprimidos.

CONCLUSIONES PRINCIPALES

La trehalasa ácida está involucrada en la hidrólisis de la trehalosa externa por C. glabrata, y la enzima también juega un papel importante en la virulencia durante la infectividad.

Título traducido de la contribución	La trehalasa ácida secretada codificada por el gen CgATH1 está involucrada en la virulencia de Candida glabrata
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	e200401
Publicación	Memorias do Instituto Oswaldo Cruz
Volumen	115
DOI	https://doi.org/10.1590/0074-02760200401
Estado	Publicada - oct. 30 2020

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

ODS 3: Salud y bienestar

Áreas temáticas de ASJC Scopus

Microbiología (médica)

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10.1590/0074-02760200401

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@article{40c111f06f024b08958dd896b6f8f4a0,

title = "The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence",

abstract = "BACKGROUND: Candida glabrata yeast is the second cause of candidiasis worldwide. Differs from other yeasts since assimilates only glucose and trehalose (a characteristic used in rapid identification tests for this pathogen) by secreting into the medium a highly active acid trehalase encoded by the CgATH1 gene. OBJECTIVE: This study aimed to characterise the function of the acid trehalase in the physiopathology of C. glabrata. METHODS: Gene deletion was performed to obtain a mutant ath1Δ strain, and the ability of the ath1Δ strain to grow in trehalase, or the presence of trehalase activity in the ath1Δ yeast cells, was verified. We also tested the virulence of the ath1Δ strain in a murine model of infection. FINDINGS: The ath1Δ mutant strain grows normally in the presence of glucose, but loses its ability to grow in trehalose. Due to the high acid trehalase activity present in wild-type cells, the cytoplasmic neutral trehalase activity is only detected in the ath1Δ strain. We also observed a significantly lower virulence of the ath1Δ strain in a murine model of infection with either normal or immunocompromised mice. MAIN CONCLUSIONS: The acid trehalase is involved in the hydrolysis of external trehalose by C. glabrata, and the enzyme also plays a major virulence role during infectivity.",

author = "Lopes, \{Rafael G.\} and Mu{\~n}oz, \{Juli{\'a}n E.\} and Barros, \{Ludmila M.\} and Alves-Jr, \{Sergio L.\} and Taborda, \{Carlos P.\} and Stambuk, \{Boris U.\}",

note = "Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine",

year = "2020",

month = oct,

day = "30",

doi = "10.1590/0074-02760200401",

language = "English (US)",

volume = "115",

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journal = "Memorias do Instituto Oswaldo Cruz",

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TY - JOUR

T1 - The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence

AU - Lopes, Rafael G.

AU - Muñoz, Julián E.

AU - Barros, Ludmila M.

AU - Alves-Jr, Sergio L.

AU - Taborda, Carlos P.

AU - Stambuk, Boris U.

N1 - Copyright: This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine

PY - 2020/10/30

Y1 - 2020/10/30

N2 - BACKGROUND: Candida glabrata yeast is the second cause of candidiasis worldwide. Differs from other yeasts since assimilates only glucose and trehalose (a characteristic used in rapid identification tests for this pathogen) by secreting into the medium a highly active acid trehalase encoded by the CgATH1 gene. OBJECTIVE: This study aimed to characterise the function of the acid trehalase in the physiopathology of C. glabrata. METHODS: Gene deletion was performed to obtain a mutant ath1Δ strain, and the ability of the ath1Δ strain to grow in trehalase, or the presence of trehalase activity in the ath1Δ yeast cells, was verified. We also tested the virulence of the ath1Δ strain in a murine model of infection. FINDINGS: The ath1Δ mutant strain grows normally in the presence of glucose, but loses its ability to grow in trehalose. Due to the high acid trehalase activity present in wild-type cells, the cytoplasmic neutral trehalase activity is only detected in the ath1Δ strain. We also observed a significantly lower virulence of the ath1Δ strain in a murine model of infection with either normal or immunocompromised mice. MAIN CONCLUSIONS: The acid trehalase is involved in the hydrolysis of external trehalose by C. glabrata, and the enzyme also plays a major virulence role during infectivity.

AB - BACKGROUND: Candida glabrata yeast is the second cause of candidiasis worldwide. Differs from other yeasts since assimilates only glucose and trehalose (a characteristic used in rapid identification tests for this pathogen) by secreting into the medium a highly active acid trehalase encoded by the CgATH1 gene. OBJECTIVE: This study aimed to characterise the function of the acid trehalase in the physiopathology of C. glabrata. METHODS: Gene deletion was performed to obtain a mutant ath1Δ strain, and the ability of the ath1Δ strain to grow in trehalase, or the presence of trehalase activity in the ath1Δ yeast cells, was verified. We also tested the virulence of the ath1Δ strain in a murine model of infection. FINDINGS: The ath1Δ mutant strain grows normally in the presence of glucose, but loses its ability to grow in trehalose. Due to the high acid trehalase activity present in wild-type cells, the cytoplasmic neutral trehalase activity is only detected in the ath1Δ strain. We also observed a significantly lower virulence of the ath1Δ strain in a murine model of infection with either normal or immunocompromised mice. MAIN CONCLUSIONS: The acid trehalase is involved in the hydrolysis of external trehalose by C. glabrata, and the enzyme also plays a major virulence role during infectivity.

UR - https://www.scopus.com/pages/publications/85095569063

UR - https://www.scopus.com/pages/publications/85095569063#tab=citedBy

U2 - 10.1590/0074-02760200401

DO - 10.1590/0074-02760200401

M3 - Research Article

C2 - 33146242

AN - SCOPUS:85095569063

SN - 0074-0276

VL - 115

SP - e200401

JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

ER -

The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence

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