The secreted acid trehalase encoded by the CgATH1 gene is involved in Candida glabrata virulence

Rafael G. Lopes, Julián E. Muñoz, Ludmila M. Barros, Sergio L. Alves-Jr, Carlos P. Taborda, Boris U. Stambuk

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

BACKGROUND

Candida glabrata yeast is the second cause of candidiasis worldwide. Differs from other yeasts since assimilates only glucose and trehalose (a characteristic used in rapid identification tests for this pathogen) by secreting into the medium a highly active acid trehalase encoded by the CgATH1 gene.

OBJECTIVE

This study aimed to characterise the function of the acid trehalase in the physiopathology of C. glabrata.

METHODS

Gene deletion was performed to obtain a mutant ath1Δ strain, and the ability of the ath1Δ strain to grow in trehalase, or the presence of trehalase activity in the ath1Δ yeast cells, was verified. We also tested the virulence of the ath1Δ strain in a murine model of infection.

FINDINGS

The ath1Δ mutant strain grows normally in the presence of glucose, but loses its ability to grow in trehalose. Due to the high acid trehalase activity present in wild-type cells, the cytoplasmic neutral trehalase activity is only detected in the ath1Δ strain. We also observed a significantly lower virulence of the ath1Δ strain in a murine model of infection with either normal or immunocompromised mice.

MAIN CONCLUSIONS

The acid trehalase is involved in the hydrolysis of external trehalose by C. glabrata, and the enzyme also plays a major virulence role during infectivity.
Idioma originalInglés estadounidense
Páginas (desde-hasta)e200401
PublicaciónMemorias do Instituto Oswaldo Cruz
Volumen115
DOI
EstadoPublicada - oct 30 2020

All Science Journal Classification (ASJC) codes

  • Microbiología (médica)

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