TY - JOUR
T1 - The eukaryotic genome, its reads, and the unfinished assembly
AU - Muñoz, José Fernando
AU - Gallo, Juan Esteban
AU - Misas, Elizabeth
AU - McEwen, Juan Guillermo
AU - Clay, Oliver Keatinge
PY - 2013/7/11
Y1 - 2013/7/11
N2 - In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.
AB - In recent years, readily affordable short read sequences provided by next-generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest in the utility of NGS-only approaches for exploring eukaryotic genomes. The concept of a static, 'finished' genome assembly, which still appears to be a faraway goal for many eukaryotes, is yielding to new paradigms. We here motivate an object-view concept where the raw reads are the main, fixed object, and assemblies with their annotations take a role of dynamically changing and modifiable views of that object.
UR - http://www.scopus.com/inward/record.url?scp=84879691390&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879691390&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2013.05.048
DO - 10.1016/j.febslet.2013.05.048
M3 - Article
C2 - 23727201
AN - SCOPUS:84879691390
SN - 0014-5793
VL - 587
SP - 2090
EP - 2093
JO - FEBS Letters
JF - FEBS Letters
IS - 14
ER -