The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis

Lissa Cruz-Saavedra, Andrea Díaz-Roa, María A. Gaona, Mónica L. Cruz, Martha Ayala, Jesús A. Cortés-Vecino, Manuel A. Patarroyo, Felio J. Bello

Resultado de la investigación: Contribución a RevistaArtículo

3 Citas (Scopus)

Resumen

© 2016 Elsevier B.V.This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future.
Idioma originalEnglish (US)
Páginas (desde-hasta)280-289
Número de páginas10
PublicaciónActa Tropica
DOI
EstadoPublished - dic 1 2016

Huella dactilar

Leishmania
Ulcer
Parasites
Mesocricetus
Coinfection
Biopsy
Cutaneous Leishmaniasis
Therapeutics
Granuloma
Bacterial Infections
Cricetinae
Cicatrix

Citar esto

Cruz-Saavedra, L., Díaz-Roa, A., Gaona, M. A., Cruz, M. L., Ayala, M., Cortés-Vecino, J. A., ... Bello, F. J. (2016). The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis. Acta Tropica, 280-289. https://doi.org/10.1016/j.actatropica.2016.09.020
Cruz-Saavedra, Lissa ; Díaz-Roa, Andrea ; Gaona, María A. ; Cruz, Mónica L. ; Ayala, Martha ; Cortés-Vecino, Jesús A. ; Patarroyo, Manuel A. ; Bello, Felio J. / The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis. En: Acta Tropica. 2016 ; pp. 280-289.
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The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis. / Cruz-Saavedra, Lissa; Díaz-Roa, Andrea; Gaona, María A.; Cruz, Mónica L.; Ayala, Martha; Cortés-Vecino, Jesús A.; Patarroyo, Manuel A.; Bello, Felio J.

En: Acta Tropica, 01.12.2016, p. 280-289.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - The effect of Lucilia sericata- and Sarconesiopsis magellanica-derived larval therapy on Leishmania panamensis

AU - Cruz-Saavedra, Lissa

AU - Díaz-Roa, Andrea

AU - Gaona, María A.

AU - Cruz, Mónica L.

AU - Ayala, Martha

AU - Cortés-Vecino, Jesús A.

AU - Patarroyo, Manuel A.

AU - Bello, Felio J.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - © 2016 Elsevier B.V.This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future.

AB - © 2016 Elsevier B.V.This study's main objective was to evaluate the action of larval therapy derived from Lucilia sericata and Sarconesiopsis magellanica (blowflies) regarding Leishmania panamensis using an in vivo model. Eighteen golden hamsters (Mesocricetus auratus) were used; they were divided into 6 groups. The first three groups consisted of 4 animals each; these, in turn, were internally distributed into subgroups consisting of 2 hamsters to be used separately in treatments derived from each blowfly species. Group 1 was used in treating leishmanial lesions with larval therapy (LT), whilst the other two groups were used for evaluating the used of larval excretions and secretions (ES) after the ulcers had formed (group 2) and before they appeared (group 3). The three remaining groups (4, 5 and 6), consisting of two animals, were used as controls in the experiments. Biopsies were taken for histopathological and molecular analysis before, during and after the treatments; biopsies and smears were taken for assessing parasite presence and bacterial co-infection. LT and larval ES proved effective in treating the ulcers caused by the parasite. There were no statistically significant differences between the blowfly species regarding the ulcer cicatrisation parameters. There were granulomas in samples taken from lesions at the end of the treatments. The antibacterial action of larval treatment regarding co-infection in lesions caused by the parasite was also verified. These results potentially validate effective LT treatment against cutaneous leishmaniasis aimed at using it with humans in the future.

U2 - 10.1016/j.actatropica.2016.09.020

DO - 10.1016/j.actatropica.2016.09.020

M3 - Article

C2 - 27686957

SP - 280

EP - 289

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

ER -