The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

Luisa Del Río-Ospina, Sara Cecilia Soto-De León, Milena Camargo, Darwin Andrés Moreno-Pérez, Ricardo Sánchez, Antonio Pérez-Prados, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

13 Citas (Scopus)

Resumen

© 2015 applies to the data made available in this article, unless otherwise stated.Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.
Idioma originalEnglish (US)
PublicaciónBMC Cancer
DOI
EstadoPublished - mar 5 2015

Huella dactilar

Cervical Intraepithelial Neoplasia
Viral Load
DNA
Human papillomavirus 16
Human papillomavirus 31
Odds Ratio
Colposcopy
Papillomavirus Infections
Colombia
Viral DNA
Uterine Cervical Neoplasms
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Infection

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Del Río-Ospina, L., Soto-De León, S. C., Camargo, M., Moreno-Pérez, D. A., Sánchez, R., Pérez-Prados, A., ... Patarroyo, M. A. (2015). The DNA load of six high-risk human papillomavirus types and its association with cervical lesions. BMC Cancer. https://doi.org/10.1186/s12885-015-1126-z
Del Río-Ospina, Luisa ; Soto-De León, Sara Cecilia ; Camargo, Milena ; Moreno-Pérez, Darwin Andrés ; Sánchez, Ricardo ; Pérez-Prados, Antonio ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / The DNA load of six high-risk human papillomavirus types and its association with cervical lesions. En: BMC Cancer. 2015.
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title = "The DNA load of six high-risk human papillomavirus types and its association with cervical lesions",
abstract = "{\circledC} 2015 applies to the data made available in this article, unless otherwise stated.Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95{\%}CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95{\%}CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95{\%}CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.",
author = "{Del R{\'i}o-Ospina}, Luisa and {Soto-De Le{\'o}n}, {Sara Cecilia} and Milena Camargo and Moreno-P{\'e}rez, {Darwin Andr{\'e}s} and Ricardo S{\'a}nchez and Antonio P{\'e}rez-Prados and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
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Del Río-Ospina, L, Soto-De León, SC, Camargo, M, Moreno-Pérez, DA, Sánchez, R, Pérez-Prados, A, Patarroyo, ME & Patarroyo, MA 2015, 'The DNA load of six high-risk human papillomavirus types and its association with cervical lesions', BMC Cancer. https://doi.org/10.1186/s12885-015-1126-z

The DNA load of six high-risk human papillomavirus types and its association with cervical lesions. / Del Río-Ospina, Luisa; Soto-De León, Sara Cecilia; Camargo, Milena; Moreno-Pérez, Darwin Andrés; Sánchez, Ricardo; Pérez-Prados, Antonio; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

En: BMC Cancer, 05.03.2015.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - The DNA load of six high-risk human papillomavirus types and its association with cervical lesions

AU - Del Río-Ospina, Luisa

AU - Soto-De León, Sara Cecilia

AU - Camargo, Milena

AU - Moreno-Pérez, Darwin Andrés

AU - Sánchez, Ricardo

AU - Pérez-Prados, Antonio

AU - Patarroyo, Manuel Elkin

AU - Patarroyo, Manuel Alfonso

PY - 2015/3/5

Y1 - 2015/3/5

N2 - © 2015 applies to the data made available in this article, unless otherwise stated.Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.

AB - © 2015 applies to the data made available in this article, unless otherwise stated.Background: Analysing human papillomavirus (HPV) viral load is important in determining the risk of developing cervical cancer (CC); most knowledge to date regarding HPV viral load and cervical lesions has been related to HPV-16. This study evaluated the association between the viral load of the six most prevalent high-risk viral types in Colombia and cervical intraepithelial neoplasia (CIN) frequency. Methods: 114 women without CIN and 59 women having CIN confirmed by colposcopy, all of them positive by conventional PCR for HPV infection in the initial screening, were included in the study. Samples were tested for six high-risk HPV types to determine viral copy number by real-time PCR. Crude and adjusted odds ratios (ORa) were estimated for evaluating the association between each viral type's DNA load and the risk of cervical lesions occurring. Results: The highest viral loads were identified for HPV-33 in CIN patients and for HPV-31 in patients without lesions (9.33 HPV copies, 2.95 interquartile range (IQR); 9.41 HPV copies, 2.58 IQR). Lesions were more frequent in HPV-16 patients having a low viral load (3.53 ORa, 1.16-10.74 95%CI) compared to those having high HPV-16 load (2.62 ORa, 1.08-6.35 95%CI). High viral load in HPV-31 patients was associated with lower CIN frequency (0.34 ORa, 0.15-0.78 95%CI). Conclusions: An association between HPV DNA load and CIN frequency was seen to be type-specific and may have depended on the duration of infection. This analysis has provided information for understanding the effect of HPV DNA load on cervical lesion development.

U2 - 10.1186/s12885-015-1126-z

DO - 10.1186/s12885-015-1126-z

M3 - Article

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

ER -

Del Río-Ospina L, Soto-De León SC, Camargo M, Moreno-Pérez DA, Sánchez R, Pérez-Prados A y otros. The DNA load of six high-risk human papillomavirus types and its association with cervical lesions. BMC Cancer. 2015 mar 5. https://doi.org/10.1186/s12885-015-1126-z