The diagnostic performance of classical molecular tests used for detecting human papillomavirus

Marina Munoz, Milena Camargo, Sara C. Soto-De Leon, Adriana Rojas-Villarraga, Ricardo Sanchez, Camilo Jaimes, Antonio Perez-Prados, Manuel E. Patarroyo, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

11 Citas (Scopus)

Resumen

Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. © 2012 Elsevier B.V..
Idioma originalEnglish (US)
Páginas (desde-hasta)32-38
Número de páginas7
PublicaciónJournal of Virological Methods
DOI
EstadoPublished - oct 1 2012

Huella dactilar

Polymerase Chain Reaction
Human papillomavirus 11
Papanicolaou Test
Colposcopy
DNA
Early Detection of Cancer
Uterine Cervical Neoplasms
Cell Biology
Disease Progression
Population

Citar esto

Munoz, Marina ; Camargo, Milena ; Soto-De Leon, Sara C. ; Rojas-Villarraga, Adriana ; Sanchez, Ricardo ; Jaimes, Camilo ; Perez-Prados, Antonio ; Patarroyo, Manuel E. ; Patarroyo, Manuel A. / The diagnostic performance of classical molecular tests used for detecting human papillomavirus. En: Journal of Virological Methods. 2012 ; pp. 32-38.
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abstract = "Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5{\%} with HC2 and 55.2{\%} with PCR. MY09/11 detected HPV-DNA in 38{\%} of samples, GP5+/6+ in 19.1{\%} and pU1M/2R in 16.4{\%}. pU1M/2R and HC2 had the highest concordance (75.31{\%}, k= 0.39 in the whole population; 74.1{\%}, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. {\circledC} 2012 Elsevier B.V..",
author = "Marina Munoz and Milena Camargo and {Soto-De Leon}, {Sara C.} and Adriana Rojas-Villarraga and Ricardo Sanchez and Camilo Jaimes and Antonio Perez-Prados and Patarroyo, {Manuel E.} and Patarroyo, {Manuel A.}",
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Munoz, M, Camargo, M, Soto-De Leon, SC, Rojas-Villarraga, A, Sanchez, R, Jaimes, C, Perez-Prados, A, Patarroyo, ME & Patarroyo, MA 2012, 'The diagnostic performance of classical molecular tests used for detecting human papillomavirus', Journal of Virological Methods, pp. 32-38. https://doi.org/10.1016/j.jviromet.2012.05.023

The diagnostic performance of classical molecular tests used for detecting human papillomavirus. / Munoz, Marina; Camargo, Milena; Soto-De Leon, Sara C.; Rojas-Villarraga, Adriana; Sanchez, Ricardo; Jaimes, Camilo; Perez-Prados, Antonio; Patarroyo, Manuel E.; Patarroyo, Manuel A.

En: Journal of Virological Methods, 01.10.2012, p. 32-38.

Resultado de la investigación: Contribución a RevistaArtículo

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T1 - The diagnostic performance of classical molecular tests used for detecting human papillomavirus

AU - Munoz, Marina

AU - Camargo, Milena

AU - Soto-De Leon, Sara C.

AU - Rojas-Villarraga, Adriana

AU - Sanchez, Ricardo

AU - Jaimes, Camilo

AU - Perez-Prados, Antonio

AU - Patarroyo, Manuel E.

AU - Patarroyo, Manuel A.

PY - 2012/10/1

Y1 - 2012/10/1

N2 - Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. © 2012 Elsevier B.V..

AB - Cervical samples were evaluated for human papillomavirus (HPV) presence using the hybrid capture-2 (HC2) assay and the polymerase chain reaction (PCR) with three different primer sets (GP5+/6+, MY09/11 and pU1M/2R). PCR results were compared to HC2 and results of all assays were compared to cytological and colposcopy findings. Post-test probability was assessed in individual assays and test combinations. HPV-DNA prevalence was 36.5% with HC2 and 55.2% with PCR. MY09/11 detected HPV-DNA in 38% of samples, GP5+/6+ in 19.1% and pU1M/2R in 16.4%. pU1M/2R and HC2 had the highest concordance (75.31%, k= 0.39 in the whole population; 74.1%, k= 0.5 in women with abnormal cytology). pU1M/2R had the best diagnostic performance, including optimal post-test probabilities and cervical abnormality detection (individually or in a panel of tests). Women positive for pU1M/2R may be at higher risk of disease progression; the assay performance when combined with a Pap smear in cervical cancer screening programs should be evaluated. © 2012 Elsevier B.V..

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JO - Journal of Virological Methods

JF - Journal of Virological Methods

SN - 0166-0934

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