The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys

Jose Rojas Caraballo; Gabriela Delgado; Raul Rodriguez; Manuel A. Patarroyo

doi:10.1016/j.vaccine.2006.12.041

The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys

Título traducido de la contribución: Antigenicidad de un fragmento recombinante de la proteína-1 de unión a reticulocitos de Plasmodium vivax (PvRBP1) en humanos y estudios de inmunogenicidad y protección en monos Aotus

Jose Rojas Caraballo
, Gabriela Delgado
, Raul Rodriguez
, Manuel A. Patarroyo

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

14 Citas (Scopus)

Resumen

Se ha descubierto que los merozoitos de Plasmodium vivax invaden específicamente los glóbulos rojos inmaduros (reticulocitos) y esta preferencia se ha asociado a dos proteínas denominadas proteína-1 y proteína-2 de unión a reticulocitos (PvRBP1 y PvRBP2). Ensayos previos de unión a reticulocitos utilizando péptidos sintéticos de 15-mer que abarcaban toda la secuencia de PvRBP1 han mostrado que 25 de los 195 péptidos sintetizados (agrupados en 4 regiones diferentes) mostraban una alta afinidad de unión a reticulocitos. En el presente estudio se expresó como proteína recombinante la región III de PvRBP1 (aminoácidos 1998-2348), que engloba 9 de los péptidos de alta afinidad de unión a reticulocitos descritos anteriormente. Esta proteína ha demostrado ser antigénica en humanos y también ha sido capaz de inducir buenas respuestas inmunitarias humorales y celulares en monos Aotus nancymaae. A pesar de su elevada inmunogenicidad, no se observó eficacia protectora en los animales inmunizados.

Título traducido de la contribución	Antigenicidad de un fragmento recombinante de la proteína-1 de unión a reticulocitos de Plasmodium vivax (PvRBP1) en humanos y estudios de inmunogenicidad y protección en monos Aotus
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	3713-3721
Número de páginas	9
Publicación	Vaccine
Volumen	25
N.º	18
DOI	https://doi.org/10.1016/j.vaccine.2006.12.041
Estado	Publicada - may 4 2007
Publicado de forma externa	Sí

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

ODS 3: Salud y bienestar

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Inmunología

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@article{5cc44c2cfe844aa69e601853116b808a,

title = "The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys",

abstract = "Plasmodium vivax merozoites have been found to specifically invade immature red blood cells (reticulocytes) and this preference has been associated with two proteins named reticulocyte binding protein-1 and protein-2 (PvRBP1 and PvRBP2). Previous reticulocyte binding assays using 15-mer synthetic peptides spanning the entire PvRBP1 sequence have shown that 25 out of the 195 peptides synthesised (grouped into 4 different regions) displayed high affinity binding to reticulocytes. The PvRBP1 region III (amino acids 1998-2348), encompassing 9 of the previously described high-affinity reticulocyte binding peptides, was expressed as a recombinant protein in the present study. This protein has been shown to be antigenic in humans and it has also been able to induce good humoral and cellular immune responses in Aotus nancymaae monkeys. Despite its high immunogenicity, no protective efficacy was observed in the immunised animals. {\textcopyright} 2006 Elsevier Ltd. All rights reserved.",

author = "\{Rojas Caraballo\}, Jose and Gabriela Delgado and Raul Rodriguez and Patarroyo, \{Manuel A.\}",

year = "2007",

month = may,

day = "4",

doi = "10.1016/j.vaccine.2006.12.041",

language = "English (US)",

volume = "25",

pages = "3713--3721",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier Limited",

number = "18",

}

The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys. / Rojas Caraballo, Jose; Delgado, Gabriela; Rodriguez, Raul et al.
En: Vaccine, Vol. 25, N.º 18, 04.05.2007, p. 3713-3721.

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys

AU - Rojas Caraballo, Jose

AU - Delgado, Gabriela

AU - Rodriguez, Raul

AU - Patarroyo, Manuel A.

PY - 2007/5/4

Y1 - 2007/5/4

N2 - Plasmodium vivax merozoites have been found to specifically invade immature red blood cells (reticulocytes) and this preference has been associated with two proteins named reticulocyte binding protein-1 and protein-2 (PvRBP1 and PvRBP2). Previous reticulocyte binding assays using 15-mer synthetic peptides spanning the entire PvRBP1 sequence have shown that 25 out of the 195 peptides synthesised (grouped into 4 different regions) displayed high affinity binding to reticulocytes. The PvRBP1 region III (amino acids 1998-2348), encompassing 9 of the previously described high-affinity reticulocyte binding peptides, was expressed as a recombinant protein in the present study. This protein has been shown to be antigenic in humans and it has also been able to induce good humoral and cellular immune responses in Aotus nancymaae monkeys. Despite its high immunogenicity, no protective efficacy was observed in the immunised animals. © 2006 Elsevier Ltd. All rights reserved.

AB - Plasmodium vivax merozoites have been found to specifically invade immature red blood cells (reticulocytes) and this preference has been associated with two proteins named reticulocyte binding protein-1 and protein-2 (PvRBP1 and PvRBP2). Previous reticulocyte binding assays using 15-mer synthetic peptides spanning the entire PvRBP1 sequence have shown that 25 out of the 195 peptides synthesised (grouped into 4 different regions) displayed high affinity binding to reticulocytes. The PvRBP1 region III (amino acids 1998-2348), encompassing 9 of the previously described high-affinity reticulocyte binding peptides, was expressed as a recombinant protein in the present study. This protein has been shown to be antigenic in humans and it has also been able to induce good humoral and cellular immune responses in Aotus nancymaae monkeys. Despite its high immunogenicity, no protective efficacy was observed in the immunised animals. © 2006 Elsevier Ltd. All rights reserved.

U2 - 10.1016/j.vaccine.2006.12.041

DO - 10.1016/j.vaccine.2006.12.041

M3 - Research Article

C2 - 17240494

SN - 0264-410X

VL - 25

SP - 3713

EP - 3721

JO - Vaccine

JF - Vaccine

IS - 18

ER -

The antigenicity of a Plasmodium vivax reticulocyte binding protein-1 (PvRBP1) recombinant fragment in humans and its immunogenicity and protection studies in Aotus monkeys

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Áreas temáticas de ASJC Scopus

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