Th1/Th2 cytokines in patients with systemic lupus erythematosus: Is tumor necrosis factor α protective?

Diana Gómez, Paula A. Correa, Luis Miguel Gómez, José Cadena, José F. Molina, Juan Manuel Anaya

Resultado de la investigación: Contribución a RevistaArtículo

126 Citas (Scopus)

Resumen

Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23%), 20 patients (34.5%), and 20 patients (34.5%) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P <.03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P <.01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P <.01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P <.04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P <.01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P <.01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production). © 2004 Elsevier Inc. All rights reserved.
Idioma originalEnglish (US)
Páginas (desde-hasta)404-413
Número de páginas10
PublicaciónSeminars in Arthritis and Rheumatism
DOI
EstadoPublished - jun 1 2004

Huella dactilar

Systemic Lupus Erythematosus
Tumor Necrosis Factor-alpha
Interleukin-10
Cytokines
Interleukin-12
Interleukin-4
Interferon-gamma
Interleukins
Autoimmunity
Interferons
DNA
Interferon-alpha
Autoantibodies
Enzyme-Linked Immunosorbent Assay
Antibodies

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Gómez, Diana ; Correa, Paula A. ; Gómez, Luis Miguel ; Cadena, José ; Molina, José F. ; Anaya, Juan Manuel. / Th1/Th2 cytokines in patients with systemic lupus erythematosus: Is tumor necrosis factor α protective?. En: Seminars in Arthritis and Rheumatism. 2004 ; pp. 404-413.
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title = "Th1/Th2 cytokines in patients with systemic lupus erythematosus: Is tumor necrosis factor α protective?",
abstract = "Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23{\%}), 20 patients (34.5{\%}), and 20 patients (34.5{\%}) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P <.03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P <.01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P <.01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P <.04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P <.01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P <.01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production). {\circledC} 2004 Elsevier Inc. All rights reserved.",
author = "Diana G{\'o}mez and Correa, {Paula A.} and G{\'o}mez, {Luis Miguel} and Jos{\'e} Cadena and Molina, {Jos{\'e} F.} and Anaya, {Juan Manuel}",
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doi = "10.1016/j.semarthrit.2003.11.002",
language = "English (US)",
pages = "404--413",
journal = "Seminars in Arthritis and Rheumatism",
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publisher = "W.B. Saunders Ltd",

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Th1/Th2 cytokines in patients with systemic lupus erythematosus: Is tumor necrosis factor α protective? / Gómez, Diana; Correa, Paula A.; Gómez, Luis Miguel; Cadena, José; Molina, José F.; Anaya, Juan Manuel.

En: Seminars in Arthritis and Rheumatism, 01.06.2004, p. 404-413.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Th1/Th2 cytokines in patients with systemic lupus erythematosus: Is tumor necrosis factor α protective?

AU - Gómez, Diana

AU - Correa, Paula A.

AU - Gómez, Luis Miguel

AU - Cadena, José

AU - Molina, José F.

AU - Anaya, Juan Manuel

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23%), 20 patients (34.5%), and 20 patients (34.5%) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P <.03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P <.01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P <.01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P <.04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P <.01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P <.01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production). © 2004 Elsevier Inc. All rights reserved.

AB - Objectives To determine the circulating levels of Th1 and Th2 cytokines in patients with systemic lupus erythematosus (SLE) and to elucidate their association with disease activity and autoimmune response. Methods We included 52 patients and 25 healthy controls. Serum levels of tumor necrosis factor (TNF) α, interferon (IFN) γ, interleukin (IL)-12p70, IL-10, and IL-4, as well as anti-DNA, -Ro, -La, -RNP, and -Sm antibodies were determined by enzyme-linked immunosorbent assay. Disease activity was recorded according to the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI) and classified as very active (SLEDAI ≥ 13), moderately active (SLEDAI: 3-12), or inactive (SLEDAI ≤ 2). Results The mean age of the patients was 34.2 ± 12.6 years, and the mean duration of disease was 4.9 ± 7.6 years. Twelve patients (23%), 20 patients (34.5%), and 20 patients (34.5%) had highly, moderately, and inactive SLE, respectively. Levels of IFN-γ, TNF-α, and IL-12 were significantly higher in patients than in healthy controls (P <.03), as well as the IL-12/IL-10, IL-12/IL-4, IFN/IL-10, IFN/IL-4, TNF/IL-10, and TNF/IL-4 ratios (P <.01), suggesting a major participation of Th1 over Th2 cytokines. Nevertheless, a direct correlation between Th1 (IFN-γ and TNF-α) and Th2 (IL-4 and IL-10) cytokines was observed in patients (r > .5, P <.01), indicating a mutual Th1-Th2 participation. TNF-α levels and the TNF/IL-10 ratio were higher in patients with inactive disease compared with patients with very active disease and controls (P <.04). IL-12 levels and IL-12/IL-4, as well as IL-12/IL-10, ratios were higher in patients with very active disease than in those with inactive SLE and controls (P <.01). IL-10 levels were associated with anti-DNA, anti-Ro, and anti-La response (P <.01). Conclusion Our results suggest that TNF-α could be a protective factor in SLE patients, whereas IL-12p70 participates in disease activity and IL-10 influences the autoimmune response (autoantibody production). © 2004 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.semarthrit.2003.11.002

DO - 10.1016/j.semarthrit.2003.11.002

M3 - Article

SP - 404

EP - 413

JO - Seminars in Arthritis and Rheumatism

JF - Seminars in Arthritis and Rheumatism

SN - 0049-0172

ER -