Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

Elizabeth Torres-Morales, Laura Taborda, Nestor Cardona, Alejandra De-la-Torre, Juan Carlos Sepulveda-Arias, Manuel Alfonso Patarroyo, Jorge Enrique Gomez-Marin

Resultado de la investigación: Contribución a RevistaArtículo

20 Citas (Scopus)

Resumen

We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.
Idioma originalEnglish (US)
Páginas (desde-hasta)315-322
Número de páginas8
PublicaciónMedical Microbiology and Immunology
DOI
EstadoPublished - ene 1 2014

Huella dactilar

Ocular Toxoplasmosis
Toxoplasmosis
Peptides
Toxoplasma
Antigens
Congenital Toxoplasmosis
T-Lymphocytes
Asymptomatic Infections
Interleukin-10
Lymphocytes
Cytokines
Proteins

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Torres-Morales, Elizabeth ; Taborda, Laura ; Cardona, Nestor ; De-la-Torre, Alejandra ; Sepulveda-Arias, Juan Carlos ; Patarroyo, Manuel Alfonso ; Gomez-Marin, Jorge Enrique. / Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis. En: Medical Microbiology and Immunology. 2014 ; pp. 315-322.
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title = "Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis",
abstract = "We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.",
author = "Elizabeth Torres-Morales and Laura Taborda and Nestor Cardona and Alejandra De-la-Torre and Sepulveda-Arias, {Juan Carlos} and Patarroyo, {Manuel Alfonso} and Gomez-Marin, {Jorge Enrique}",
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Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis. / Torres-Morales, Elizabeth; Taborda, Laura; Cardona, Nestor; De-la-Torre, Alejandra; Sepulveda-Arias, Juan Carlos; Patarroyo, Manuel Alfonso; Gomez-Marin, Jorge Enrique.

En: Medical Microbiology and Immunology, 01.01.2014, p. 315-322.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Th1 and Th2 immune response to P30 and ROP18 peptides in human toxoplasmosis

AU - Torres-Morales, Elizabeth

AU - Taborda, Laura

AU - Cardona, Nestor

AU - De-la-Torre, Alejandra

AU - Sepulveda-Arias, Juan Carlos

AU - Patarroyo, Manuel Alfonso

AU - Gomez-Marin, Jorge Enrique

PY - 2014/1/1

Y1 - 2014/1/1

N2 - We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.

AB - We determined the specific lymphocyte proliferative response and cytokine profile production regarding Toxoplasma P30 (2017 from virulent and non-virulent strain) and ROP18 protein-derived peptides (from clonal lineages I, II and III) in 19 patients having ocular toxoplasmosis, five suffering chronic asymptomatic infection, nine with congenital toxoplasmosis and eight Toxoplasma negative people. A Beckman Coulter FC500 flow cytometer was used for determining antigen-specific T cells (CD3+ CD4+ or CD3+ CD8+ cells) in peripheral blood culture. IFN γ and IL10 levels were determined in culture supernatants. Specific CD4+ and CD8+ T cell response to total antigen and P30- and ROP18-derived peptides was observed in infected people. Ocular toxoplasmosis patients had a preferential Th2 response after antigenic stimulation. Non-virulent peptide 2017 was able to shift response toward Th1 in congenitally infected children and virulent peptide 2017 induced a Th2 response in chronically infected, asymptomatic people. An immune response in human toxoplasmosis after ex vivo antigenic stimulation was Th1- or Th2-skewed, depending on a patient’s clinical condition. Colombian ocular toxoplasmosis patients’ immune response was Th2-skewed, regardless of the nature of antigen stimulus.

U2 - 10.1007/s00430-014-0339-0

DO - 10.1007/s00430-014-0339-0

M3 - Article

SP - 315

EP - 322

JO - Medical Microbiology and Immunology

JF - Medical Microbiology and Immunology

SN - 0300-8584

ER -