Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Karen Orjuela; Catalina Amador; Manuel A. Patarroyo

doi:10.1016/j.diagmicrobio.2007.04.003

Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Título traducido de la contribución: Genes sintéticos: una herramienta para identificar genotipos del virus del papiloma humano mediante hibridación y ensayos basados en la reacción en cadena de la polimerasa

Karen Orjuela
, Catalina Amador
, Manuel A. Patarroyo

Producción científica: Contribución a revista › Estudio breve › revisión exhaustiva

3 Citas (Scopus)

Resumen

La obtención de controles positivos de la reacción en cadena de la polimerasa (PCR) para las pruebas de diagnóstico del virus del papiloma humano (VPH) ha resultado difícil debido a la variación de la prevalencia en las distintas regiones geográficas de cada tipo vírico de alto riesgo. Se diseñaron oligonucleótidos superpuestos para secuencias específicas de tipo (TS) de VPH-18, VPH-31, VPH-45 y VPH-58. Se construyeron genes virales sintéticos del VPH mediante PCR de ensamblaje en 2 pasos para diagnosticar con precisión la infección por VPH TS.

Título traducido de la contribución	Genes sintéticos: una herramienta para identificar genotipos del virus del papiloma humano mediante hibridación y ensayos basados en la reacción en cadena de la polimerasa
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	101-104
Número de páginas	4
Publicación	Diagnostic Microbiology and Infectious Disease
Volumen	59
N.º	1
DOI	https://doi.org/10.1016/j.diagmicrobio.2007.04.003
Estado	Publicada - sept 1 2007
Publicado de forma externa	Sí

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

ODS 3: Salud y bienestar

Áreas temáticas de ASJC Scopus

Inmunología

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10.1016/j.diagmicrobio.2007.04.003

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@article{a93a61bc5a124e5ca1d2287afa45898a,

title = "Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays",

abstract = "Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. {\textcopyright} 2007 Elsevier Inc. All rights reserved.",

author = "Karen Orjuela and Catalina Amador and Patarroyo, \{Manuel A.\}",

year = "2007",

month = sep,

day = "1",

doi = "10.1016/j.diagmicrobio.2007.04.003",

language = "English (US)",

volume = "59",

pages = "101--104",

journal = "Diagnostic Microbiology and Infectious Disease",

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}

Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays. / Orjuela, Karen; Amador, Catalina; Patarroyo, Manuel A.
En: Diagnostic Microbiology and Infectious Disease, Vol. 59, N.º 1, 01.09.2007, p. 101-104.

Producción científica: Contribución a revista › Estudio breve › revisión exhaustiva

TY - JOUR

T1 - Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

AU - Orjuela, Karen

AU - Amador, Catalina

AU - Patarroyo, Manuel A.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. © 2007 Elsevier Inc. All rights reserved.

AB - Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. © 2007 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.diagmicrobio.2007.04.003

DO - 10.1016/j.diagmicrobio.2007.04.003

M3 - Short survey

C2 - 17512155

SN - 0732-8893

VL - 59

SP - 101

EP - 104

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays

Resumen

ODS de las Naciones Unidas

Áreas temáticas de ASJC Scopus

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