Synthetic evaluation of standard and microwave-assisted solid phase peptide synthesis of a long chimeric peptide derived from four Plasmodium falciparum proteins

Yahson F. Varela, Magnolia Vanegas Murcia, Manuel Elkin Patarroyo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

4 Citas (Scopus)

Resumen

An 82-residue-long chimeric peptide was synthesised by solid phase peptide synthesis (SPPS), following the Fmoc protocol. Microwave (MW) radiation-assisted synthesis was compared to standard synthesis using low loading (0.20 mmol/g) of polyethylene glycol (PEG) resin. Similar synthetic difficulties were found when the chimeric peptide was obtained via these two reaction conditions, indicating that such difficulties were inherent to the sequence and could not be resolved using MW; by contrast, the number of coupling cycles and total reaction time became reduced whilst crude yield and percentage recovery after purification were higher for MW radiation-assisted synthesis.

Idioma originalInglés estadounidense
Número de artículo2877
PublicaciónMolecules
Volumen23
N.º11
DOI
EstadoPublicada - nov 5 2018

All Science Journal Classification (ASJC) codes

  • Química analítica
  • Química (miscelánea)
  • Medicina molecular
  • Ciencias farmacéuticas
  • Descubrimiento de medicamentos
  • Química física y teórica
  • Química orgánica

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