TY - JOUR
T1 - Staurosporine-induced apoptosis in P388D1 macrophages involves both extrinsic and intrinsic pathways
AU - Nakamura-López, Yuko
AU - Sarmiento-Silva, Rosa Elena
AU - Moran-Andrade, Julio
AU - Gómez-García, Beatriz
N1 - Funding Information:
This work was supported by the Consejo Nacional de Ciencia y Tecnología (CONACyT; grant # 43944-M) and by the Dirección General de Asuntos del Personal Academico-Universidad Nacional Autonoma de Mexico (DGAPA-UNAM; grant # IN2004007). We thank Andi Espinoza-Sanchez and Berenice Hernández for the technical assistance, Rocio Tirado Mendoza, PhD, for helpful discussions, and Veronica Yakoleff for English revision and for editing of the manuscript. This work is part of the PhD dissertation submitted by Y. Nakamura-Lopez in partial fulfillment of the degree requirements.
PY - 2009/9
Y1 - 2009/9
N2 - Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.
AB - Treatment of P388D1, a macrophage-like cell line, with staurosporine triggered apoptosis through the activation of caspase-9 and caspase-3. Unexpected effects of staurosporine on the induction of apoptosis were the activation of caspase-8, and an increase of the levels of TNF-α. The increased TNF-α levels led to activation of caspase-8 by an autocrine effect via the TNF receptor expressed by the P388D1 macrophages. In contrast, P388D1 macrophages that either had been exposed to UV light or treated with dexamethasone did not undergo apoptosis.
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U2 - 10.1016/j.cellbi.2009.06.010
DO - 10.1016/j.cellbi.2009.06.010
M3 - Research Article
C2 - 19524691
AN - SCOPUS:68949213602
SN - 1065-6995
VL - 33
SP - 1026
EP - 1031
JO - Cell Biology International
JF - Cell Biology International
IS - 9
ER -
